Sequences from confirmation amplicon


Confirmation sequences for line 695B02 and hit At5g65080 are shown.
Line ID 695B02
Locus At5g65080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25998721
Primers used for PCR KN37 - 8474, show primer details
   
Sequence Name 61-K103224-0022-695-B02C-KN37
GenBank Accession LN510490 [GenBank]
Sequencing primer gene specific primer, KN37: 5'-ACTCCTTCAACAATAAGCCAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K103224-0022-695-B02C-KN37
AAAATGATAGGACTGAGCCCATCAAGCCCAGAAAGACACACCACAAAAGCTAACTTAGCT
TATGTATGATGGCGCCTTTAGGTCTTAAAAGGCATTATAGTTTGGTCTCCTCCTACCATA
TGTGATTTTTTTTTTTTTTAGGGAAACCAACATTTTGATCACAAATTCAACCCAACTTCG
GGATGTCCGGGATCTATGGCAAAATTTCAAAAAAAAAAGAAAGTTGGGTTCATTTGATCA
CCAGGTTGTTAACCCCCCCCAAAATGATTTGAAAACTATGCAATTAAAAAAACACATAAA
AACCACAATCTCAGGCAAAAACCCTTCTTTATTTGGCCCCTAAATTAAAGTATCAAATTA
AAGGACCTCCCAGGGAAACTAGCTCCAACGGGATTTTAACTATTTTAAACCTTGAACCAA
AAAAAAAAAAGTTCTTTAAGAAAAATCTATAATTGGGGCAGGGAATTTGCCAAATAAACA
TTTCTGCTGGCAGGATCAAGTATAAGTAAAGTTTCTATAAAGGCAATGGTAAAAACGTTA
ATTTTTTTGGAGTTAGTGACAAGGACATCCCCTTTTCTCCTTTATGGAGGGAAAAAACCA
GGTAACAGGAATGGAACAAAATTGATTTAGAGGGGGTCGGTACCTCTTTTTGGCCCAGGA
AACCCAATATCTCCAAAACAACGGACTAATTAAAAGGCGGGATATTTCCATTTGTAAATG
GCTCCAGGTCCGGGAAATCTACTGGGATCACCATTGAGAATAAGGGTCAATATGGAGAAA
AAAAAAAAGTAATTACCAATTTTTTTT
   
Sequence Name 29-K103225-0022-695-B02C-8409
GenBank Accession LN499242 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K103225-0022-695-B02C-8409
ATTTACAATTGAATATATCCTGCATTTTAATTAGTCAGTCGTTTTGGAGATATTGGGTCT
CATGGGCCAAAAAGAGGTACCGACCCTCTCTATATCAATTTTGTTCAATTCATGTTACTT
GCTTTTTTCCCTCTATAATGTAGAAATGGGGATGTCCTTGTCACTAACTCAATATATATA
AACGTTCTTACCATTGCCTTCATAGAAACTCTACTAATACTTGATCATGCAAGCAGAAAT
GTTAATTTGGCAACTACCATGCACAAATTATAGATATTACTAATAGAACTTTTTTTTATT
TGGTTCAAGGTTTAAAATAGTTAAAATCCAGTTGTAGCTAGTTTCCTTGGTAGCTCCTTT
AATTTGATACTTTAATTTAGTGGCAATATACAGAAGTGTTTTCGCTTGAGATTGTGGTTC
TTATGTGTCTTTCTAATTGCATAGTTTACAACTCATTTAGGATGGAGTTAACAACATGCT
GATCAAATGAACCAAACTTACTCTATTATCTGAAATCTTGCAATAGATCACGGACATCAC
GAAGTAGGATTGAATTTGTGATCAAAATGTTGGTATCCATAAAAAAAAAAAAAATCACAT
ATGGTGGGAGGAAACCAAACTATAAGGCCTTTAAAAACCTAAGGGCCCCTTCAAACAAAA
CCAAATTAAGCTTTGGGGGTGTGTCTTTCTGGGCTTGATGGGGCCCAATCCAATAATTTT
TTTCACCTTCTCAAAAAGGCTAAACCCTTTTCCTGGG

Last Updated on 06.12.2018 16:11