Sequences from confirmation amplicon


Confirmation sequences for line 695F08 and hit At5g08000 are shown.
Line ID 695F08
Locus At5g08000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2564949
Primers used for PCR CQ96 - 8474, show primer details
   
Sequence Name 46-K107133-0022-695-F08C-CQ96
GenBank Accession LN505877 [GenBank]
Sequencing primer gene specific primer, CQ96: 5'-CATTCCCTTCTAGTGCTAGGTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K107133-0022-695-F08C-CQ96
TTATTTCTTATGGCTTATCTANAATTTGAAGGATACCATTTATTTTTTTTTGGACAAAGA
AGGATAGCCATTTAATACTACACTTCAATGTTGGATTAACTAACTTATTATGCCTATTTA
ATGGTCTAAACTTTAGATAACATCTACAACAATTCAATTATGCCTTTTTTTGGGGATTGT
ATAAAGTTTGAACCTATGGTTAGTGTATTGACTTGCGCGTCTCTTTGCAACGGTTTTATC
TTGTTGCTGTAAGCTATTCTTTGAAAACACATTAATGATAAATAAATTGAAAAGTATAGA
GATGGCAATTGTTTCAAAAGCTAATCTTTCTGTTTGATAATACTTTACATGAAAAACAAA
AAATTAAGAATAGTTTCAAACAATACAACTTGTTTACCTTGTCCTAACAAATTCAACTCA
AATGACATGTGTTTGCTTTAAAATAGTAACAACTGTAAATTCATTTGCTCTTGAAACATA
AACAATACTGTTGACTGCAAGTCTCTGTAATGATCTTTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 38-K107132-0022-695-F08C-8409
GenBank Accession LN502999 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K107132-0022-695-F08C-8409
GCCATTTACAATTGAAAGATCATTACAGAGACTTGCAGTCAACAGTATTGTTTATGTTTC
AAGAGCAAATGAATTTACAGTTGTTACTATTTTAAAGCAAACACATGTCATTTGAGTTGA
ATTTGTTAGGACAAGGTAAACAAGTTGTATTGTTTGAAACTATTCTTAATTTTTTGTTTT
TCATGTAAAGTATTATCAAACAGAAAGATTAGCTTTTGAAACAATTGCCATCTCTATACT
TTTCAATTTATTTATCATTAATGTGTTTTCAAAGAATAGCTTACAGCAACAAGATAAAAC
CGTTGCAAAGAGACGCGCAAGTCAATACACTAACCATAGGTTCAAACTTTATACAATCCC
CAAAAAAAGGCATAATTGAATTGTTGTAGATGTTATCTAAAGTTTAGACCATTAAATAGG
CATAATAAGTTAGTTAATCCAACATTGAAGTGTAGTATTAAATGGCTATCCTTCTTTGTC
CAAAAAAAAATAAATGGTATCCTTCAAATTTCTAGATAAAGCCATTAAGAAAATAAATGT
GTAGAGAAGCAAAGAGCCGTACCTAGCACTAGAAAGGGGAAT

Last Updated on 06.12.2018 16:11