Sequences from confirmation amplicon


Confirmation sequences for line 695G07 and hit At1g63940 are shown.
Line ID 695G07
Locus At1g63940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23730011
Primers used for PCR IW62 - 8474, show primer details
   
Sequence Name 43-K033213-022-695-G07C-IW62
GenBank Accession LN504745 [GenBank]
Sequencing primer gene specific primer, IW62: 5'-AAGGTGCGTAAGCCTAACAATAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K033213-022-695-G07C-IW62
CACATCAACAAGTAAACCATAGTGTTACTGATGAAGAAACACTAAGGGATCGATTTGAAT
CACCTCTTTGGTCACAATGCATAGCCGACCATCAGCCATTCCATTTTCCACAAAAGTTCT
AGCAGCATAACCAGCAGCATTTCCTCCACCAACAATCACAAACCTAACACAATCACACAA
TCAATCGCATAGAAATTCATCAACGCCAGAATCAAAATTGGAAGAATTATATATACGAAC
TCGCGATTCTCGTTAGCGAACGAAGCAGTGACGAGGCTTCTGGAAGCGATTCTAGAACCG
ATCGGAGAAAAACGAGCGGGAAATCGGCGAGCGAGAGACGGAGAAGACGGACACCAAAGA
GATAATCCGGACTTCGTCGGCAACGTGGTTGATGCTAACGCCATGACTCTACGAACTGTA
ACAACAGATTAGAGAGATAAGATTTCGATCTAGGGTTTTGACAATCGAGGAAGAAGATGG
TGGTGAGTGAGAGAGATAAGAAGACCTGCAGACATTAGTTTATAAGAAGAGCCAAATTCA
GATTTAATTATTGAAACAAGTGTGTGAGTCTCTCTGTAGTGTTGGCTCTATAACAAAGCA
CTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
C
   
Sequence Name 35-K033213-022-695-G07C-8409
GenBank Accession LN501728 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K033213-022-695-G07C-8409
TGCTTTGTTATAGAGCCAACACTACAGAGAGACTCACACACTTGTTTCAATAATTAAATC
TGAATTTGGCTCTTCTTATAAACTAATGTCTGCAGGTCTTCTTATCTCTCTCACTCACCA
CCATCTTCTTCCTCGATTGTCAAAACCCTAGATCGAAATCTTATCTCTCTAATCTGTTGT
TACAGTTCGTAGAGTCATGGCGTTAGCATCAACCACGTTGCCGACGAAGTCCGGATTATC
TCTTTGGTGTCCGTCTTCTCCGTCTCTCGCTCGCCGATTTCCCGCTCGTTTTTCTCCGAT
CGGTTCTAGAATCGCTTCCAGAAGCCTCGTCACTGCTTCGTTCGCTAACGAGAATCGCGA
GTTCGTATATATAATTCTTCCAATTTTGATTCTGGCGTTGATGAATTTCTATGCGATTGA
TTGTGTGATTGTGTTAGGTTTGTGATTGTTGGTGGAGGAAATGCTGCTGGTTATGCTGCT
AGAACTTTTGTGGAAAATGGAATGGCTGATGGTCGGCTATGCATTGTGACCAAAGAGGTG
ATTCAAATCGATCCCTTAGTGTTTTCTTCATCAGTAACACTATTGGTTTTACTTTGTTTG
ATTGTTGGATTTGTGGTTGTTATTGTTAGGCTTACGCACC

Last Updated on 06.12.2018 16:11