Sequences from confirmation amplicon


Confirmation sequences for line 695G12 and hit At4g19038 are shown.
Line ID 695G12
Locus At4g19038 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10430278
Primers used for PCR 43qv - 8474, show primer details
   
Sequence Name 54-K115837-0022-695-G12C-43qv
GenBank Accession LN508585 [GenBank]
Sequencing primer gene specific primer, 43qv: 5'-GTTGTGGAAACATAAACGGAGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K115837-0022-695-G12C-43qv
TAACATGTTTTATTAACGGTTATTCTTATAATAAATATAAATATGACTTCATGGCTTTTC
ACTAGTGAAATACAAGTATTAAGAGCGTTTGCAAGCATACTGGCAGATGCAAGCGGAGCG
ATAATCGGGGCCTTCGAGGCAACCACCCTTGCCATGTCTCTTGAGTGCACATGCAAAGGC
ACATGCGTCGTATTTACATTCTTCCGGCCATAGGACCTCCTTACACTCTACCATTTCCTC
AAGTGGTTGTCCCTTTGCCAGACATATCTCCCCTATTACAGTAGATTTAATCTACATATC
TCCTCAATTATTGAATATATCCGGACATGTAGATTTCATTGTCGTTTTTTCCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCA
   
Sequence Name 22-K115833-0022-695-G12C-8409
GenBank Accession LN496256 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115833-0022-695-G12C-8409
CATTTCATTGAATATATCCTGGAAAAACGACAATGAAATCTACATGTCCGGATATATTCA
ATAATTGAGGAGATATGTAGATTAAATCTACTGTAATAGGGGAGATATGTCTGGCAAAGG
GACAACCACTTGAGGAAATGGTAGAGTGTAAGGAGGTCCTATGGCCGGAAGAATGTAAAT
ACGACGCATGTGCCTTTGCATGTGCACTCAAGAGACATGGCAAGGGTGGTTGCCTCGAAG
GCCCCGATTATCGCTCCGCTTGCATCTGCCAGTATGCTTGCAAACGCTCTTAATACTTGT
ATTTCACTAGTGAAAAGCCATGAAGTCATATTTATATTTATTATAAGAATAACCGTTTAA
TAAAAACATTGTTTTATCGTGTGCTAGACATTTTATCATTCTCCGTTTATTTNTCCACAA

Last Updated on 06.12.2018 16:11