Sequences from confirmation amplicon


Confirmation sequences for line 696A02 and hit At1g62380 are shown.
Line ID 696A02
Locus At1g62380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23082638
Primers used for PCR CQ97 - 8474, show primer details
   
Sequence Name 23-K107199-0022-696-A02C-CQ97
GenBank Accession LN496580 [GenBank]
Sequencing primer gene specific primer, CQ97: 5'-TTAAAGAAAGGAGATGGTAGGGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K107199-0022-696-A02C-CQ97
TCACGAACTCTTGTTTCTTTACTTTAACTTCTTCTTTTTTAACAATTTGAATGAAACATA
TTTATACATCTTATCTATGACTATGACTCGCACATATTTTCTCAGAATTTTATTTATTTT
ATTGAAAGCCAATCTCTCTCATTTGCGCCTATAAATAGCAACCCTCTCTCATTCTACAAG
TCTCACATCAAAGCAATTAACAAATACACAACTTGTAACTTCAAATAGATTACTTTCAAA
GAAGAGAGAGAGAAAGAAAGATGGAGAAGAACATGAAGTTTCCAGTAGTAGACTTGTCCA
AGCTCAATGGGGAAGAGAGAGACCAAACCATGGCTCTAATCAATGAAGCTTGTGAGAATT
GGGGCTTCTTTGAGGTACATACACAATATCTAACATATTATTGTTTGAAAAGATTTGACT
ATAGCTAGCTCTTAGTGGTAAGGTTGATCTTTTGTTAATTTCATTTTTCAGATAGTGAAC
CATGGATTACCACATGACTTAATGGACAAGATCGAGAAGATGACAAAGGACCATTACAAG
ACATGCCAAGAACAAAAGTTCAATGACATGCTCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGGGTTTATT
   
Sequence Name 07-K107198-0022-696-A02C-8409
GenBank Accession LN489297 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K107198-0022-696-A02C-8409
TTGACTTTTGTTCTTGGCATGTCTTGTAATGGTCCTTTGTCATCTTCTCGATCTTGTCCA
TTAAGTCATGTGGTAATCCATGGTTCACTATCTGAAAAATGAAATTAACAAAAGATCAAC
CTTACCACTAAGAGCTAGCTATAGTCAAATCTTTTCAAACAATAATATGTTAGATATTGT
GTATGTACCTCAAAGAAGCCCCAATTCTCACAAGCTTCATTGATTAGAGCCATGGTTTGG
TCTCTCTCTTCCCCATTGAGCTTGGACAAGTCTACTACTGGAAACTTCATGTTCTTCTCC
ATCTTTCTTTCTCTCTCTCTTCTTTGAAAGTAATCTATTTGAAGTTACAAGTTGTGTATT
TGTTAATTGCTTTGATGTGAGACTTGTAGAATGAGAGAGGGTTGCTATTTATAGGCGCAA
ATGAGAGAGATTGGCTTTCAATAAAATAAATAAAATTCTGAGAAAATATGTGCGAGTCAT
AGTCATAGATAAGATGTATAAATATGTTTCATTCAAATTGTTAAAAAAGAAGAAGTTAAA
GTAAAAGAAACAAGAGTTCGTGGAGCATGTATGTGAGCACATCATAGATGGACCCCTACC
ATCTCCNTTCTTTA

Last Updated on 06.12.2018 16:11