Sequences from confirmation amplicon


Confirmation sequences for line 696G03 and hit At1g68600 are shown.
Line ID 696G03
Locus At1g68600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25759979
Primers used for PCR 31fm - 8474, show primer details
   
Sequence Name 81-K111396-0022-696-G03C-31fm
GenBank Accession LN514085 [GenBank]
Sequencing primer gene specific primer, 31fm: 5'-CAATCCAAGTAACAAACAAGCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K111396-0022-696-G03C-31fm
ATACAGTAAAATGTAACTCAATAAGTAAATCCGAACCCATCAAAATGAAAGATGGTATCC
AAAAAAAGGAACAGTCTTAGGATTAAAAGAATCTTTACTAGATTTGCTTCACATGCGCAA
GTGTCTTGAGTTCAAGATTCCAACAAAACATAAAAACAAAACTCAAAGATCCAATCAAAG
GATCATAAACTAGGCTATGAGTTAAGAATCAATACCGACGTAATACTCGAAAATGAGAAC
GACGGTGAGAATCGCCCAAACTGCGAATTTACTAGCATCTTGAAGCGGCTCTTTGAGAAA
GATGACGAAAGAACAGAGTGCCAAAGCAATTCCCATCTTGATGGAGAAGTAAAGTTTCCT
ACGATCAGAATGTCCCAGCGCGTACAACTTCGCCGGAGCCTCGTAGAGAGCCCTCCACGA
GCAACACCGGAACTTGGAGTCACGGCTATCAGCGACGTCGCTTGACTGCCAGAGAAGCTT
CTCTGTGTTTTGCTCCGGTACTGATCCCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCACGATTATT
   
Sequence Name 74-K111395-0022-696-G03C-8409
GenBank Accession LN513097 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K111395-0022-696-G03C-8409
AAACACAGAGAAGCTTCTCTGGCAGTCAAGCGACGTCGCTGATAGCCGTGACTCCAAGTT
CCGGTGTTGCTCGTGGAGGGCTCTCTACGAGGCTCCGGCGAAGTTGTACGCGCTGGGACA
TTCTGATCGTAGGAAACTTTACTTCTCCATCAAGATGGGAATTGCTTTGGCACTCTGTTC
TTTCGTCATCTTTCTCAAAGAGCCGCTTCAAGATGCTAGTAAATTCGCAGTTTGGGCGAT
TCTCACCGTCGTTCTCATTTTCGAGTATTACGTCGGTATTGATTCTTAACTCATAGCCTA
GTTTATGATCCTTTGATTGGATCTTTGAGTTTTGTTTTTATGTTTTGTTGGAATCTTGAA
CTCAAGACACTTGCGCATGTGAAGCAAATCTAGTAAAGATTCTTTTAATCCTAAGACTGT
TCCTTTTTTTGGATTCCATCTTTCATTTTGATGGGTTCGGATTTACTTATTGAGTTACAT
TTTACTGTATTATTCACATTTTCAGACCAACTCTTATACTTTCTTTGCTTGTTTGTTACT
TGGATTG

Last Updated on 06.12.2018 16:11