Sequences from confirmation amplicon


Confirmation sequences for line 697F07 and hit At3g45640 are shown.
Line ID 697F07
Locus At3g45640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16757458
Primers used for PCR CL31 - 8474, show primer details
   
Sequence Name 12-K028744-022-697-F07C-8409
GenBank Accession LN491434 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K028744-022-697-F07C-8409
AAAACTACCGAAGATATCGTAACTTATGAAACCGTCCTCCGTGAGTTTCCACCGCCGGAA
AAATCCGTGTATTGACCACCGCCGGTGTTCATTTCTCTCTCAATTGATCAAAGTCGAGAT
T
   
Sequence Name 12-K028744-022-697-F07C-CL31
GenBank Accession LN491435 [GenBank]
Sequencing primer gene specific primer, CL31: 5'-TCTTCTTCTTCATCTTCTTCCTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K028744-022-697-F07C-CL31
CTCATCTCAGTCTCTCTGTTGACTCCGACGTGTTATATAAAACCATACAAATACCTTCAG
ATTCACTACTTCAAACCTCAAAGCAACCACTGAATCTCGACTTTGATCAATTGAGAGAGA
AATGAACACCGGCGGTGGCCAATACACGGATTTTCCGGCGGTGGAAACTCACGGAGGACA
GTTCATAAGTTACGATATCTTCGGTAGTTTATTCGAGATCACATCTAAGTATCGTCCTCC
GATTATACCAATTGGTCGTGGAGCTTATGGAATCGTTTGGTATGTTTCCTTCTCTGAGCT
TTTTCTTAAGTTTTATTCTTTGAGATCGGAGTTTTTAATTTCTGGATTTTGATTTTGTGA
AGCTCTGTGTTGGATACGGAGACGAACGAGCTAGTAGCGATGAAGAAGATAGCTAATGCT
TTTGATAATCATATGGATGCTAAGCGTACGCTTCGTGAGATCAAGCTTCTTCGTCATCTT
GATCATGAAAACGTTAGTGTTTTTGTTCGATTTTGTGAAATATCTGTAGAAATTAGTAAA
TAAATCATGATTAGGGTTTTTTGATTTGGTATTGCAACAGATTATAGCTATAAGAGATGT
TGTTCCACCACCACTAAGAAGACAGTTCAGTGATGTTTATATCTCTACTGAATTAATGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCACTGAGTATG
ATGGTCAATA

Last Updated on 06.12.2018 16:11