Sequences from confirmation amplicon


Confirmation sequences for line 697H10 and hit At4g16295 are shown.
Line ID 697H10
Locus At4g16295 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9216125
Primers used for PCR CM53 - 8474, show primer details
   
Sequence Name 34-K104125-0022-697-H10C-CM53
GenBank Accession LN501395 [GenBank]
Sequencing primer gene specific primer, CM53: 5'-TCCCAAAACACGTTTACATTCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K104125-0022-697-H10C-CM53
ATAGCACCAAAAAAAGTTGAGTGAAGCATATTTTCTCCGAAGTTCCAAGAAAATCTATTT
CTAAACTTCAGATTAATTTCACCTAGATCATCTTCTTTAGATTTACAATGGATGAAAAGA
GTTTCACCAGTTGTCAAACCGTTAACAACCGTGACTTGCCACTCAGAAATCTTCGGGACT
ATAATGTCTCTCGTGGTGGTGCCGCCGACTAAGACATGGTCTTGACATGTTAGGGCCAGA
GAAAAGCAAATTGCCAAGAGAAATTGTTTGAGAAATTGACAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCCGCA
   
Sequence Name 50-K104126-0022-697-H10C-8409
GenBank Accession LN507151 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K104126-0022-697-H10C-8409
TTACATTGAATATATCCTGTCAATTTCTCAAACAATTTCTCTTGGCAATTTGCTTTTCTC
TGGCCCTAACATGTCAAGACCATGTCTTAGTCGGCGGCACCACCACGAGAGACATTATAG
TCCCGAAGATTTCTGAGTGGCAAGTCACGGTTGTTAACGGTTTGACAACTGGTGAAACTC
TTTTCATCCATTGTAAATCTAAAGAAGATGATCTAGGTGAAATTAATCTGAAGTTTAGAA
ATAGATTTTCTTGGAACTTCGGAGAAAATATGCTTCACTCAACTTTTTTTTGGTGCTATA
TGAACAAAGATAATGGTCATATGAATGTAAACTGATTTTGGGA

Last Updated on 06.12.2018 16:11