Sequences from confirmation amplicon


Confirmation sequences for line 698F12 and hit At5g05350 are shown.
Line ID 698F12
Locus At5g05350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1586173
Primers used for PCR EP74 - 8474, show primer details
   
Sequence Name 19-K103006-0022-698-F12C-8409
GenBank Accession LN494717 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K103006-0022-698-F12C-8409
CATTTACACGTCGTGACTTGGGCAAGCATGGTTCAACATACCAGTCATAACCATGAATAG
AATCGCACCGGAGACAGCAACCACAAAGATCCACAAAAACATAACCATGTTCAACGGGTT
CTGTAGCCATTGCTTACCCATTTTCATCAAACAAGCCCACTCGATTTTCCGGGAAGACAC
CACATCGTGGCTGCTAGAGCTTGAGCTGTTGACAGAGCGTGAAACTTCATCTCTTTCCTC
AGCCATTCTCTTGAGTCTAGCAGAAGGGGAACCAAATTTCAAAAGCTTAATCCTATTAGA
GACATCAGGCAACGAACTCTCGGACCATCTCCTAGGTTTCTCTTCACTGATGAGAATCTT
ATGAGATGTTGAGAGAGCAGGAGGACATGAAACCCTAGTCTTAGATATCTCAACCTCATC
AACTGAA
   
Sequence Name 67-K103007-0022-698-F12C-EP74
GenBank Accession LN511789 [GenBank]
Sequencing primer gene specific primer, EP74: 5'-AATTAATGGGTTCAGTTGATGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K103007-0022-698-F12C-EP74
TCCTCCTGCTCTCTCACATCTCATAAGATTCTCATCAGTGAAGAGAAACCTAGGAGATGG
TCCGAGAGTTCGTTGCCTGATGTCTCTAATAGGATTAAGCTTTTGAAATTTGGTTCCCCT
TCTGCTAGACTCAAGAGAATGGCTGAGGAAAGAGATGAAGTTTCACGCTCTGTCAACAGC
TCAAGCTCTAGCAGCCACGATGTGGTGTCTTCCCGGAAAATCGAGTGGGCTTGTTTGATG
AAAATGGGTAAGCAATGGCTACAGAACCCGTTGAACATGGTTATGTTTTTGTGGATCTTT
GTGGTTGCTGTCTCCGGTGCGATTCTATTCATGGTTATGACTGGTATGTTGAACCATGCT
TTGCCCAAGTCACGACGTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCTACATTCACGTCCAAATGGG

Last Updated on 06.12.2018 16:11