Sequences from confirmation amplicon


Confirmation sequences for line 699A06 and hit At1g48480 are shown.
Line ID 699A06
Locus At1g48480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17919486
Primers used for PCR EU40 - 8474, show primer details
   
Sequence Name 01-K103490-0022-699-A06C-EU40
GenBank Accession LN486539 [GenBank]
Sequencing primer gene specific primer, EU40: 5'-TGTTAAACACATTGTCCCTACCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K103490-0022-699-A06C-EU40
GTATTATGGAACATATCAATGTTAAGAATCAATGATCAAAGAGATTAAGCACTAAACTTA
ACCATAATCTAAATGTATTCAAGCCTATCTATAGGCACTAAAAGCATTGACTTGCCTCAA
AAGAAACCAAACTCAATCTAAGCACAAGTTTCGATATGAACTCACCATGTAAGAGAGCTG
ATAAGCTTCCCATAGGCATGAAGTCATAGACAAGAAGTTTCTCATCTCTGCTGAAATAGT
AAGCTCTCAATGGCACCAAGTTCTCATGATCCATCGCACCAACCAACTCAATCTTCTCCT
TAAACTCTTTATCAGCCATCATCACATCCTTCAACCTCTTCACCGCCACAACCGTCACCG
CGTCAAGCACTGCCTTATACGCCGTCCCAAACGTTCCTTTCCCAAGAACCTCCGCAGAAG
CTCTCAACAAGTCTTCAAGATCAAAAACCTTAGTCGCGTTACCAAAGAACACAAGCTTCT
TCGTCGCTGGACCGTTCCCTTCACTGGCTTTTCCGTTTCCAGTGAAGCAAACGACAAAAA
TTGTCAATTGAATAACATCAAATCATATATCCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 25-K103491-0022-699-A06C-8409
GenBank Accession LN497403 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K103491-0022-699-A06C-8409
CGGACTGAGCCTTTACATTGAATATATCCTGGATATATGATTTGATGTTATTCAATTGAC
AATTTTTGTCGTTTGCTTCACTGGAAACGGAAAAGCCAGTGAAGGGAACGGTCCAGCGAC
GAAGAAGCTTGTGTTCTTTGGTAACGCGACTAAGGTTTTTGATCTTGAAGACTTGTTGAG
AGCTTCTGCGGAGGTTCTTGGGAAAGGAACGTTTGGGACGGCGTATAAGGCAGTGCTTGA
CGCGGTGACGGTTGTGGCGGTGAAGAGGTTGAAGGATGTGATGATGGCTGATAAAGAGTT
TAAGGAGAAGATTGAGTTGGTTGGTGCGATGGATCATGAGAACTTGGTGCCATTGAGAGC
TTACTATTTCAGCAGAGATGAGAAACTTCTTGTCTATGACTTCATGCCTATGGGAAGCTT
ATCAGCTCTCTTACATGGTGAGTTCATATCGAAACTTGTGCTTAGATTGAGTTTGGTTTC
TTTTGAGGCAAGTCAATGCTTTTAGTGCCTATAGATAGGCTTGAATACATTTAGATTATG
GTTAAGTTTAGTGCTTAATCTCTTTGATCATTGATTCTTAACATTGATATGATTTCCATA
ATACTTTGTATAGTTTCTGTGAATTGGTAGGGACAAT

Last Updated on 06.12.2018 16:11