Sequences from confirmation amplicon


Confirmation sequences for line 699B05 and hit At5g22510 are shown.
Line ID 699B05
Locus At5g22510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7476976
Primers used for PCR 25yz - 8474, show primer details
   
Sequence Name 32-K123356-0022-699-B05C-25yz
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 25yz: 5'-GCTAAACAAAAATGGGATATAGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K123356-0022-699-B05C-25yz
TCTGTAATAGAGCGAAATTTGGAAAAAAGATGACATTTTTCTATGATTTGCGATGTCTGA
TTCATTCATTCCCACATGTTTAGGTTCTCTTGTGTAAAGTTTGTTCCTTTCTTATGTCTT
CTTATCCAATTTTTGCAGGTATCATTTTAAAGTTTGATCATTTCTTCTTTGGGTATATAG
GGTAGTATTCAAAATGGCAGCTTCAGAAACAGTTCTACGTGTTCCATTAGGATCTGTATC
TCAATCTTGCTATCTAGCTTCCTTCTTTGTTAACTCAACACCAAACCTCTCGTTTAAACC
CGTTAGTAGAAACAGAAAGACCGTCCGGTGTACAAACTCGCATGAGGTCTCAAGCGTACC
AAAACATTCATTTCATAGTTCAAACTCTGTTTTGAAAGGTAAAAAGTTTGTTTCCACGAT
ATGCAAATGCCAAAAACATGATGTTGAGGAGAGTATTAGATCAACGTTACTTCCATCTGA
CGGTTTGAGCAGTGAATTGAAAAGTGACTTAGATGAAATGCCTTTACCTGTTAATGGAAG
TGTTTCATCTAATGGTAATGCACAATCAGTTGGAACAAAGTCTATTGAGGATGAAGCTTG
GGATCTGTTGAGGCAGTCTGTTGTTTTCTATTGTGGAAGTCCTATTGGAACTATTGCTGC
TAATGATCCGAACTCTACTAGTGTTTTGAATTATGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATT
   
Sequence Name 15-K123355-0022-699-B05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K123355-0022-699-B05C-8409
CATTTAATTGAATATATCATAATTCAAACACTAGTAGAGTTCGGATCATTAGCAGCAATA
GTTCCAATAGGACTTCCACAATAGAAAACAACAGACTGCCTCAACAGATCCCAAGCTTCA
TCCTCAATAGACTTTGTTCCAACTGATTGTGCATTACCATTAGATGAAACACTTCCATTA
ACAGGTAAAGGCATTTCATCTAAGTCACTTTTCAATTCACTGCTCAAACCGTCAGATGGA
AGTAACGTTGATCTAATACTCTCCTCAACATCATGTTTTTGGCATTTGCATATCGTGGAA
ACAAACTTTTTACCTTTCAAAACAGAGTTTGAACTATGAAATGAATGTTTTGGTACGCTT
GAGACCTCATGCGAGTTTGTACACCGGACGGTCTTTCTGTTTCTACTAACGGGTTTAAAC
GAGAGGTTTGGTGTTGAGTTAACAAAGAAGGAAGCTAGATAGCAAGATTGAGATACAGAT
CCTAATGGAACACGTAGAACTGTTTCTGAAGCTGCCATTTTGAATACTACCCTATATACC
CAAAGAAGAAATGATCAAACTTTAAAATGATACCTGCAAAAATTGGATAAGAAGACATAA
GAAAGGAACAAACTTTACACAAGAGAACCTAAACATGTGGGAATGAATGAATCAGACATC
GCAAATCATAGAAAAATGTCATCTTTTTTCCAAATTTCGCTCTAATTACAGAGAAACACT
GTTGATCAAGTCCAATTTCAGTGCTTTGTCGCTATATCCCATTTTTGTTTAG

Last Updated on 06.12.2018 16:11