Sequences from confirmation amplicon


Confirmation sequences for line 699C04 and hit At1g67720 are shown.
Line ID 699C04
Locus At1g67720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25390678
Primers used for PCR EU41 - 8474, show primer details
   
Sequence Name 12-K103500-0022-699-C04C-EU41
GenBank Accession LN491541 [GenBank]
Sequencing primer gene specific primer, EU41: 5'-AGAAGATTTTGGTCCTGAACTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K103500-0022-699-C04C-EU41
TCTATCTTCTGTCCATTTTCACCTTTGACCGTTTAAAGTACTACATGAACAACATCAAGA
TTCACAAACCACATATCCTCTCTATGCTTTTCTTGTTCACCAACAGGCAAGATCGCTGAT
CCGTAAAGGAGACGTGTGCGGGATCATAGATCCATGTATAGCAAGCAACGTGAAGATCGA
GTCAGTCTGGAGAGTTGCAGAGGTCGCAAACCAATGTGTTGAGCAGCGTGGACATAACAG
GCCAAGGATGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGA
   
Sequence Name 28-K103501-0022-699-C04C-8409
GenBank Accession LN498807 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K103501-0022-699-C04C-8409
GCCATTTACATTGAATATATCCTGCATCCTTGGCCTGTTATGTCCACGCTGCTCAACACA
TTGGTTTGCGACCTCTGCAACTCTCCAGACTGACTCGATCTTCACGTTGCTTGCTATACA
TGGATCTATGATCCCGCACACGTCTCCTTTACGGATCAGCGATCTTGCCTGTTGGTGAAC
AAGAAAAGCATAGAGAGGATATGTGGTTTGTGAATCTTGATGTTGTTCATGTAGTACTTT
AAACGGTCAAAGGTGAAAATGGACAGAAGATTAGAGGTGTTTACCCAGTGAACTATATTC
AGTTCAGGACCAAAATCTTCTATCCTTTTTCGCCTTTGGCCGTTTGCGGTTC

Last Updated on 06.12.2018 16:11