Sequences from confirmation amplicon


Confirmation sequences for line 699F04 and hit At3g08570 are shown.
Line ID 699F04
Locus At3g08570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2602592
Primers used for PCR 46qi - 8474, show primer details
   
Sequence Name 47-K115550-0022-699-F04C-46qi
GenBank Accession LN506293 [GenBank]
Sequencing primer gene specific primer, 46qi: 5'-TTCACAAGTTCATGGCTTTGATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K115550-0022-699-F04C-46qi
GGCTATACAGAGCCATTGATATGTTTCTAAGGTAAAGTTGTTCTTCTATCCCTTAATATG
GAGGCTGTGTCTCTGTTTTTGCAACATTTTGTATTGATAGAGTGTCAATGTTCTTTGTGT
CTCTGGTTAGGGGCATCCGTCGCTGAACGAACAAGAATGTAAGAGTCTCTGCAAATTCAT
AGACACTCAGAAACTTTCACAAGAAGCATGCAACCATGTGGCTCAGAACGATCGGCTGCC
GATGCAGATGGTGGTCCGAGTCCTCTACTCAGAACAGCTGCGTATGAAGAATGTAATGTC
CGGAGAGTCAGGGGAAGGGCTATTGCTGTCTTCATCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 20-K115549-0022-699-F04C-8409
GenBank Accession LN495349 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K115549-0022-699-F04C-8409
TTGATGAAGACGCAATAGCCCTTCCCCTGACTCTCCGGACATTACATTCTTCATACGCAG
CTGTTCTGAGTAGAGGACTCGGACCACCATCTGCATCGGCAGCCGATCGTTCTGAGCCAC
ATGGTTGCATGCTTCTTGTGAAAGTTTCTGAGTGTCTATGAATTTGCAGAGACTCTTACA
TTCTTGTTCGTTCAGCGACGGATGCCCCTAACCAGAGACACAAAGAACATTGACACTCTA
TCAATACAAAATGTTGCAAAAACAGAGACACAGCCTCCATATTAAGGGATAGAAGAACAA
CTTTACCTTTAGAAACATATCAATGGCTCTGTATAGCCCATCATCCATGACACGTGCGTA
ATCAGGCAATATCTCTATCAAAGCCATGAACTTGTGA

Last Updated on 06.12.2018 16:11