Sequences from confirmation amplicon


Confirmation sequences for line 700B07 and hit At5g45780 are shown.
Line ID 700B07
Locus At5g45780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18569153
Primers used for PCR EU42 - 8474, show primer details
   
Sequence Name 13-K103500-0022-700-B07C-EU42
GenBank Accession LN492005 [GenBank]
Sequencing primer gene specific primer, EU42: 5'-ATTTTTGGGTTATTGTAGTGGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K103500-0022-700-B07C-EU42
GATGAAGATGAGAAAGAGGTTTTGTCTGGTTGGGATATTAACTCTGTTGATCCTTGTACT
TGGAACATGGTTGGTTGTTCTTCTGAAGGTTTTGTGGTTTCTCTGTGAGTATCTTTTGCA
CACCACTTGTTCGACTAAATGTCTCTGAGAAACGTTTCTTTGCTGAGTTTATTGAAATCT
TTAATGCAGAGAGATGGCTAGTAAAGGATTATCAGGGATACTATCTACTAGTATTGGGGA
ATTAACTCACAATACTAGTAGATATTATCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGAATATGTCCGCACGGTTATT
   
Sequence Name 29-K103501-0022-700-B07C-8409
GenBank Accession LN499250 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K103501-0022-700-B07C-8409
GCCATTTACAATTGAATATATCCTGATAATATCTACTAGTATTGTGAGTTAATTCCCCAA
TACTAGTAGATAGTATCCCTGATAATCCTTTACTAGCCATCTCTCTGCATTAAAGATTTC
AATAAACTCAGCAAAGAAACGTTTCTCAGAGACATTTAGTCGAACAAGTGGTGTGCAAAA
GATACTCACAGAGAAACCACAAAACCTTCAGAAGAACAACCAACCATGTTCCAAGTACAA
GGATCAACAGAGTTAATATCCCAACCAGACAAAACCTCTTTCTCATCTTTCATCTTGTTC
TTCACTGACATTAACGCAGCCACTACAATACCCCAAAA

Last Updated on 06.12.2018 16:11