Sequences from confirmation amplicon


Confirmation sequences for line 700G05 and hit At3g01280 are shown.
Line ID 700G05
Locus At3g01280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:87030
Primers used for PCR MF03 - 8474, show primer details
   
Sequence Name 27-K031693-022-700-G05C-MF03
GenBank Accession LN498263 [GenBank]
Sequencing primer gene specific primer, MF03: 5'-TGATTTGAAAGTTTGCACTGATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K031693-022-700-G05C-MF03
ATTTCTCTCCTGTTTTTCTTGTATATTGAGTTATAGTTTTTTGTTTGTTTGTTGGGCTAC
TTACCTTACAACTTTGTGTGTATTGGTTTCAGTTTCTGATCACCGCTACCGTTGATGAGG
CTGCACCCGGACTGAGGTCAATCTTCAGCTTCAAGGTTCCTGACCAAAATTCTGGCAAGG
TATATTTTATTAATTCAACAACATTTCTTTGTATTGTTTTAAAGTTTCATCAGTTGAGAG
TTGACTTCTCATTTTTATCGTGTTTCGTCTTATAAACAGGTTGAGCTGCAATACTTGCAA
CAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACATTCACGTCCAAATGG
   
Sequence Name 75-K031693-022-700-G05C-8409
GenBank Accession LN513170 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K031693-022-700-G05C-8409
ATTTACAATTGAATATTGTTGCAAGTATTGCAGCTCAACCTGTTTATAAGACGAAACACG
ATAAAAATGAGAAGTCAACTCTCAACTGATGAAACTTTAAAACAATACAAAGAAATGTTG
TTGAATTAATAAAATATACCTTGCCAGAATTTTGGTCAGGAACCTTGAAGCTGAAGATTG
ACCTCAGTCCGGGTGCAGCCTCATCAACGGTAGCGGTGATCAGAAACTGAAACCAATACA
CACAAAGTTGTAAGGTAAGTAGCCCAACAAACAAACAAAAAACTATAACTCAATATACAA
GAAAAACAGGAGATGAAATTGAGTACACAAATACACTTACAGTAGAATCAGTGCAAACTT
TCAAATC

Last Updated on 06.12.2018 16:11