Sequences from confirmation amplicon


Confirmation sequences for line 700G06 and hit At3g44250 are shown.
Line ID 700G06
Locus At3g44250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15948647
Primers used for PCR NE12 - 8474, show primer details
   
Sequence Name 79-K102370-0022-700-G06C-8409
GenBank Accession LN513807 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K102370-0022-700-G06C-8409
TTACAATTGATTATAAGGCTTCTTAACCTTCTTTACTTCTTCGATTGGAGTTTGCCCAAT
GGAATGACTATTGAAGATATTGACATGGAAGAAGACGAAGGGTTCGCTATCGCCAAGAAA
GTCCCTCTTGTTCTCATACAAACTTCTCATCGCTGGTGAAAATGTAAAAGTTTAAATAAG
ATCAAGATTTCTATATGTTTGTGTACTTTACTATCGGGTTTTGTTTTTCTTTTGTTCTTT
GTTTTACGTTTATTATGTCTTGTAATCGATATATTTCATGTTTAAAATTACTTATGTTAA
TCTTACACGTAGTTTCTTTGGGCTAGTGGTTGACCAAAGGTTCATTAATACTTCTAAACG
TAATTTCTTGTAAATTAATGGTGTAAAGCTATTCGGAAGTCCAAACTGCCGTATGTAGAG
CCGTTTGTAGTGATCTGAGTATTGTGGGAAGAGCATGTCTATCGCAAAGATGTCGTACAT
GCAAAATCGTCCGTTAAAGATCCAAATGTTG
   
Sequence Name 79-K102371-0022-700-G06C-NE12
GenBank Accession LN513808 [GenBank]
Sequencing primer gene specific primer, NE12: 5'-ACAACATTTGGATCTTTAACGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K102371-0022-700-G06C-NE12
GATAGACATGCTCTTCCCACAATACTCAGATCACTACAAACGGCTCTACATACGGCAGTT
TGGACTTCCGAATAGCTTTACACCATTAATTTACAAGAAATTACGTTTAGAAGTATTAAT
GAACCTTTGGTCAACCACTAGCCCAAAGAAACTACGTGTAAGATTAACATAAGTAATTTT
AAACATGAAATATATCGATTACAAGACATAATAAACGTAAAACAAAGAACAAAAGAAAAA
CAAAACCCGATAGTAAAGTACACAAACATATAGAAATCTTGATCTTATTTAAACTTTTAC
ATTTTCACCAGCGATGAGAAGTTTGTATGAGAACAAGAGGGACTTTCTTGGCGATAGCGA
ACCCTTCGTCTTCTTCCATGTCAATATCTTCAATAGTCATTCCATTGGGCAAACTCCAAT
CGAAGAAGTAAAGAAGGTTAAGAAGCCTTATAATCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11