Sequences from confirmation amplicon


Confirmation sequences for line 701E11 and hit At3g06260 are shown.
Line ID 701E11
Locus At3g06260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1894037
Primers used for PCR DN06 - 8474, show primer details
   
Sequence Name 30-K101829-0022-701-E11C-8409
GenBank Accession LN499656 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K101829-0022-701-E11C-8409
CATTTCATTGAATATATCCTGTGTAATGATCCTGTGTTATATCAGCGTTGCGGATCCTAT
GTCGTCTAATATTCGCCGGTGATATAAAAGCGGTTAATCATAGGTGGAACCAGCATGGTC
TAGGAGGTGATAATTTCGAAGGAAGATGTAGAACGTTGCATCCGGGACCGATAAGTCTTC
TTCACTGGAGTGGAAAAGGGAAGCCATGGTTAAGACTAGATTCAAGGAAGCCTTGTATCG
TTGATCATCTATGGGCACCGTATGATCTGTACCGTTCATCAAGACATTCATTAGAAGAGT
AGAGAGGCAAAAGAAAAAAACCATTAAACATGGCACATATAGGAGAGGAGAAGATACAAA
AGAAAGCAGGGGTTTT
   
Sequence Name 74-K101832-0022-701-E11C-DN06
GenBank Accession LN513044 [GenBank]
Sequencing primer gene specific primer, DN06: 5'-AAAAACCCCTGCTTTCTTTTGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K101832-0022-701-E11C-DN06
GTTTTTTTCTTTTGCCTCTCTACTCTTCTATGAATGTCTTGATGAACGGTACAGATCATA
CGGTGCCCATAGATGATCAACGATACAAGGCTTCCTTGAATCTAGTCTTAACCATGGCTT
CCCTTTTCCACTCCAGTGAAGAAGACTTATCGGTCCCGGATGCAACGTTCTACATCTTCC
TTCGAAATTATCACCTCCTAGACCATGCTGGTTCCACCTATGATTAACCGCTTTTATATC
ACCGGCGAATATTAGACGACATAGGATCCGCAACGCTGATATAACACAGGATCATTTACA
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGAT

Last Updated on 06.12.2018 16:11