Sequences from confirmation amplicon


Confirmation sequences for line 703A06 and hit At5g50850 are shown.
Line ID 703A06
Locus At5g50850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20689909
Primers used for PCR 60zf - 8474, show primer details
   
Sequence Name 59-K124919-0022-703-A06C-60zf
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 60zf: 5'-AAAACAGTGCCCTTTCGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K124919-0022-703-A06C-60zf
CGCCGTCTCTCCTTTTTCGCCGGCGTGGTTAGATCGTACAAAGCTCAGGTAATCAAATCT
ATCTCCGATGTTGATCTACTCTGTTTCTCCGGATTTATGATTTCTCTATTCAATTTCCGA
TGATTGTTAGATCTACGGGGCTTTTGATCCTGCGATTACTGTTGCTTTTTGTTTGTTAAT
CAGAGAGAATGTTGGGAATCTTGAGGCAAAGAGCCATCGATGGAGCTTCGGTATGAGACT
TTTTTACTCCTTTTATTTGTTTCAGTGCGATTACGATTTTGAGATTTTTACTTGGGAGAT
TTTTAGGTTCGGATTTTGTTTTCTGATTTTCGAAATTTGGTTTTTGATCAGACTCTTAGG
AGGACGCGATTCGCATTGGTTTCTGCTAGGAGCTATGCAGCTGGTGCAAAAGAGGTAAAA
TGTCTTTTATTGAATTTTCGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAAATGTCCGCAGCGTTATT
   
Sequence Name 43-K124918-0022-703-A06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K124918-0022-703-A06C-8409
TAAAGACATTTTACCTCTTTTGCACCAGCTGCATAGCTCCTAGCAGAAACCAATGCGAAT
CGCGTCCTCCTAAGAGTCTGATCAAAAACCAAATTTCGAAAATCAGAAAACAAAATCCGA
ACCTAAAAATCTCCCAAGTAAAAATCTCAAAATCGTAATCGCACTGAAACAAATAAAAGG
AGTAAAAAAGTCTCATACCGAAGCTCCATCGATGGCTCTTTGCCTCAAGATTCCCAACAT
TCTCTCTGATTAACAAACAAAAAGCAACAGTAATCGCAGGATCAAAAGCCCCGTAGATCT
AACAATCATCGGAAATTGAATAGAGAAATCATAAATCCGGAGAAACAGAGTAGATCAACA
TCGGAGATAGATTTGATTACCTGAGCTTTGTACGATCTAACCACGCCGGCGAAAAAGTGA
GAGACGGCGGATTAAGAAGAGAGCAGATACGACTCAAAGCGTGAGCGAAAGGGCACTGTT
T

Last Updated on 06.12.2018 16:11