Sequences from confirmation amplicon


Confirmation sequences for line 703C11 and hit At4g19010 are shown.
Line ID 703C11
Locus At4g19010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10412591
Primers used for PCR 22bw - 8474, show primer details
   
Sequence Name 09-K114266-0022-703-C11C-22bw
GenBank Accession LN490278 [GenBank]
Sequencing primer gene specific primer, 22bw: 5'-TTCCTTTACCTGAAAGCCTTTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114266-0022-703-C11C-22bw
ATCTTCATCAAAAAAGCAATATCCCCAGTACGTAGCCAACTATCTTCAACTATACTCATC
TGAGTTTGCTTTTGGGTTATTCAAGTATCCTGACAGACAAATGAAACGGTGTTCAGATTC
TCTCCAAGTAAAATTAAAACTGTTCCATAAAAATATGTAGCACAAAATCAAGAACAAGAT
AATAAGCCTAATCTATCAACACAAGCACCAAATAAGCCCCACGAAAGTTGGAAGATCCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 01-K114265-0022-703-C11C-8409
GenBank Accession LN486667 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K114265-0022-703-C11C-8409
CACTTTCGTGGGGCTTATTTGGTGCTTGTGTTGATAGATTAGGCTTATTATCTTGTTCTT
GATTTTGTGCTACATATTTTTATGGAACAGTTTTAATTTTACTTGGAGAGAATCTGAACA
CCGTTCATTTGTCTGTCAGGATACTTGAATAACCCAAAAGCAACTCAGATGAGTATAGTT
GAAGATAGTTGGCTACGTACTGGGGATATTGCTTATTTTGATGAAGATGGTTACTTGTTT
ATTGTTGACCGTATTAAGGAGATTATAAAGTACAAAGGCTTTCNGGTAAAGGA

Last Updated on 06.12.2018 16:11