Sequences from confirmation amplicon


Confirmation sequences for line 703D04 and hit At5g21326 are shown.
Line ID 703D04
Locus At5g21326 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7219459
Primers used for PCR 44kh - 8474, show primer details
   
Sequence Name 76-K113595-0022-703-D04C-44kh
GenBank Accession LN513434 [GenBank]
Sequencing primer gene specific primer, 44kh: 5'-TGAATTCTCACTGATTTGTCCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K113595-0022-703-D04C-44kh
CGACGATGTGTCTCATTGACTGTTTATACATATATTTAATAAGCTCTGAATACTGACCAT
TCTAGATTCTCAGCTTATCAAACTTATTTGGTCTTGGCAGGGTGATGGTCTTCTTCACAC
TGCATGCGGAACTCCAAACTATGCTGCTCCTGAGGTATGCTTAGGCATGTGTCTTGTATG
TCATGCGGTTGTTGCACCATGTTATGTTACTAATAGCTTCGTTGTTGCTCTTGTCACAAG
GTTCTTAATGATCAAGGTTATGACGGGGCAACCGCAGACTTGTGGTCCTGTGGAGTGATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGC
   
Sequence Name 34-K113594-0022-703-D04C-8409
GenBank Accession LN501487 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K113594-0022-703-D04C-8409
TCATTGAATATCACTCCACAGGACCACAAGTCTGCGGTTGCCCCGTCATAACCTTGATCA
TTAAGAACCTTGTGACAAGAGCAACAACGAAGCTATTAGTAACATAACATGGTGCAACAA
CCGCATGACATACAAGACACATGCCTAAGCATACCTCAGGAGCAGCATAGTTTGGAGTTC
CGCATGCAGTGTGAAGAAGACCATCACCCTGCCAAGACCAAATAAGTTTGATAAGCTGAG
AATCTAGAATGGTCAGTATTCAGAGCTTATTAAATATATGTATAAACAGTCAATGAGACA
CATCGTCGTTGAACTACAGGTTTCTTCATTAGAATAGGACAAGGACAAATCATGNAGAAT
TC

Last Updated on 06.12.2018 16:11