Sequences from confirmation amplicon


Confirmation sequences for line 703E02 and hit At2g43290 are shown.
Line ID 703E02
Locus At2g43290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17991541
Primers used for PCR 45kh - 8474, show primer details
   
Sequence Name 77-K113595-0022-703-E02C-45kh
GenBank Accession LN513580 [GenBank]
Sequencing primer gene specific primer, 45kh: 5'-GGAAAAACAAGAAAAACAAACCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K113595-0022-703-E02C-45kh
ACCCTAGTTAAAGATCAATTACTGCTGCTAAAGCCACCACCTTTCATCATCTGAAGAAAC
TCTTTGTAATTGACTCTACCATCACCATCTGCATCAACTTGCATAATCATCTTCTTACAA
CCATCTAGGGTCTTCCCTTGCTTGAGTCCCAAGGAAGCCATCACAGATTTCAACTCCTCC
ACAGTGATAAACCCATCTCCGTCTTGGTCAAACACGTTAAACGCATCTTTCATATCCTCT
TCCTCTGTTTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 35-K113594-0022-703-E02C-8409
GenBank Accession LN501903 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K113594-0022-703-E02C-8409
TCATTGAATTATCCTGGANACAGAGGAAGAGGATATGAAAGATGCGTTTAACGTGTTTGA
CCAAGACGGAGATGGGTTTATCACTGTGGAGGAGTTGAAATCTGTGATGGCTTCCTTGGG
ACTCAAGCAAGGGAAGACCCTAGATGGTTGTAAGAAGATGATTATGCAAGTTGATGCAGA
TGGTGATGGTAGAGTCAATTACAAAGAGTTTCTTCAGATGATGAAAGGTGGTGGCTTTAG
CAGCAGTAATTGATCTTTAACTAGGGTTTGATTTGACAGAAGAGAAGTGTGTTTATTCGA
TTGATTGGTTTGTTTTTCTTGTTTTTC

Last Updated on 06.12.2018 16:11