Sequences from confirmation amplicon


Confirmation sequences for line 703G09 and hit At5g45280 are shown.
Line ID 703G09
Locus At5g45280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18348864
Primers used for PCR 20an - 8474, show primer details
   
Sequence Name 02-K106755-0022-703-G09C-8409
GenBank Accession LN487050 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K106755-0022-703-G09C-8409
GCCTTTACATTGAACATTGGTGCCATACATTCTTGATCTGCAAAAAAAGGAATAAAATTT
TTAAATCCAAATCATATCTAGTGTTATTATTTCTACCGAAAACAATAATCATAAAAACAA
AATCGAACCTGCCAAGAATCGAAGGCGGCATTGATGACGAAAAGCGGTGTTTGTAAGGTC
TTAGCAACGTATTGAGGGAAGAAACACTGTGAAAATATAAACATAAGCGTTTATACAATA
TAACGAACTCGGAAAAATTTATGCCTTGTATATCAAGTATGTTTTCGATAAATTTACCAA
ATCAGGCTTCATACTTGAGGTGCAAGAAGCAGGTAGACTCTTTGCAGATCCCTGTAAATA
GTGATCGAGATTAGTCGTGATGGGATACACCTGATATATTAGAGAAATATGGCCAAGGAA
AAGAGAGATGTATCCTACGTGGGTCGCTACGACTTTGGCGTAGTAGGATTGGATGTAAGA
TCCTCCAGTGATATCCTTACTGTTCAAACAACAAAATAACAAAGGTTGTTATTTGTTATC
TAGGATGCTAGCAATTGTATGTGTGACTCTCTAGAAATTATGAATGTTACCCG
   
Sequence Name 18-K106756-0022-703-G09C-20an
GenBank Accession LN494323 [GenBank]
Sequencing primer gene specific primer, 20an: 5'-GTTACTTCATCCACGGGTAACATT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K106756-0022-703-G09C-20an
ATTGCTAGCATCCTAGATAACAAATAACAACCTTTGTTATTTTGTTGTTTGAACAGTAAG
GATATCACTGGAGGATCTTACATCCAATCCTACTACGCCAAAGTCGTAGCGACCCACGTA
GGATACATCTCTCTTTTCCTTGGCCATATTTCTCTAATATATCAGGTGTATCCCATCACG
ACTAATCTCGATCACTATTTACAGGGATCTGCAAAGAGTCTACCTGCTTCTTGCACCTCA
AGTATGAAGCCTGATTTGGTAAATTTATCGAAAACATACTTGATATACAAGGCATAAATT
TTTCCGAGTTCGTTATATTGTATAAACGCTTATGTTTATATTTTCACAGTGTTTCTTCCC
TCAATACGTTGCTAAGACCTTACAAACACCGCTTTTCGTCATCAATGCCGCCTTCGATTC
TTGGCAGGTTCGATTTTGTTTTTATGATTATTGTTTTCGGTAGAAATAATAACACTAGAT
ATGATTTGGATTTAAAAATTTTATTCCTTTTTTTGCAGATCAAGAATGTATTGGCACCAA
TGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TATCC

Last Updated on 06.12.2018 16:11