Sequences from confirmation amplicon


Confirmation sequences for line 705E01 and hit At1g01710 are shown.
Line ID 705E01
Locus At1g01710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:265664
Primers used for PCR CR34 - 8474, show primer details
   
Sequence Name 26-K107199-0022-705-E01C-CR34
GenBank Accession LN497953 [GenBank]
Sequencing primer gene specific primer, CR34: 5'-CTTTCAGGAGTTATCGGAACAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K107199-0022-705-E01C-CR34
AGATTCGATTTTGTGAGCCCGCAATTCAACAAATCAGACAAAGTCTCCTCCGAGGTTAAC
AACTCGCTGAAATAAATCAATTATATCATAGAGTACTCTTGAGTGTATACTCATAAATTC
CATTAAGTATTTCTTAAAAAAAAAATCAAGAAACTCACAATGCCTTCTTGCAGATTGAAC
TATTGGTTTAAAGCAAAGGGAAGGCTTTCTGATGATCAAGCTTTGCACCGGTAAGTAACT
CCATCTATGTATATCAAGGTAACCATCTAAAAATTGGTATTATTCATCTGGTAACAATCC
TTTAAACTTTAAAGGCAAGAATGATTGTAGACAAGTGAATCAATGGTGTTTTAGTATCCC
AAGTTGATAGGTTGAGACTTGTAAAAGGCCAAACTTTCTTAATAGCATTGAAAAACTGAC
GGTATATTGAAATTCAATTTATCATTCTTAGCAAACTAATCATTTGGCAAAGCCATGTTG
ATATGCAGATGTGTCGTTGCATTTGCCTCGGATTTAATATTTTGTGGTGTTGGTTTGAAC
CCTCACCGGAGAAAAGGGGTGAAATCCGCCGCACTTAGCCTGGACCATGCGTAGGTTTTT
ATTTTCTCTTGGTGATGTATGAGTTTTGTGTGTCTGGGTTAAATTTTTTAATTACATTGA
GGCATTATTCCAGTAGGGATCTTCCAGATAAGTAACTATTACGAGTTACTATTCCTCATT
TGGTTTGCAGGATGTGGTTTCACCGACCTCTAAGAGCCGACAAGTGGTTGCTCTATGTGG
TAGTTTTTTCTATCCTTGTTTCTTATGAACATTTCAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAATGT
   
Sequence Name 10-K107198-0022-705-E01C-8409
GenBank Accession LN490682 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K107198-0022-705-E01C-8409
CATTTACATTGAATTATCCTGAAATGTTCATAAGAAACAAGGATAGAAAAAACTACCACA
TAGAGCAACCACTCGTCGGCTCTTAGAGGTCGGTGAAACCACATCCTGCAAACCAAATGA
GGAATAGTAACTCGTAATAGTTACTTATCTGGAAGATCCCTACTGGAATAATGCCTCAAT
GTAATTAAAAAATTTAACCCAGACACACAAAACTCATACATCACCAAGAGAAAATAAAAA
CCTACGCATGGTCCAGGCTAAGTGCGGCGGATTTCACCCCTTTTCTCCGGTGAGGGTTCA
AACCAACACCACAAAATATTAAATCCGAGGCAAATGCAACGACACATCTGCATATCAACA
TGGCTTTGCCAAATGATTAGTTTGCTAAGAATGATAAATTGAATTTCAATATACCGTCAG
TTTTTCAATGCTATTAAGAAAGTTTGGCCTTTTACAAGTCTCAACCTATCAACTTGGGAT
ACTAAAACACCATTGATTCACTTGTCTACAATCATTCTTGCCTTTAAAGTTTAAAGGATT
GTTACCAGATGAATAATACCAATTTTTAGATGGTTACCTTGATATACATAGATGGAGTTA
CTTACCGGTGCAAAGCTTGATCATCAGAAAGCCTTCCCTTTGCTCTAAACCAATAGTTCA
ATCTGCAAGAAGGCATTGTGAGTTTCTTGATTTTTTTTTTAAAAAATACT

Last Updated on 06.12.2018 16:11