Sequences from confirmation amplicon


Confirmation sequences for line 705G02 and hit At3g19540 are shown.
Line ID 705G02
Locus At3g19540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6782051
Primers used for PCR NL37 - 8474, show primer details
   
Sequence Name 49-K104113-0022-705-G02C-NL37
GenBank Accession LN506823 [GenBank]
Sequencing primer gene specific primer, NL37: 5'-CTGCTACAAGAACCGTTAGGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K104113-0022-705-G02C-NL37
GTTTGTTCTTTGGCAGATGAATCCGACATGTGGTATGTTGAGCTTGCTGTTGGGGGTAGT
AAGGTTCGTGCTGGCTGCAATGGCAAGCTTGTATGGAGACACACTCCTTGGCTCGGATCT
CATACTGCTAAAGGACCTGTCAGACCTCTCCGCCGTGGACTTCAGGTAAAAGTCTTGACT
TTTTAGGTGTTTTAGTAGTTCGAGAGATCAAAGAGATCCTTAAGTGTTTTTTGGATGATT
CAGGGACTAGATCCGAGAACAACTGCTGCAATGTTTGCGGAAGCGAAGTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
   
Sequence Name 51-K104114-0022-705-G02C-8409
GenBank Accession LN507487 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K104114-0022-705-G02C-8409
GAGCCTTTACCTTCGCTTCCGCAAACATTGCAGCAGTTGTTCTCGGATCTAGTCCCTGAA
TCATCCAAAAAACACTTAAGGATCTCTTTGATCTCTCGAACTACTAAAACACCTAAAAAG
TCAAGACTTTTACCTGAAGTCCACGGCGGAGAGGTCTGACAGGTCCTTTAGCAGTATGAG
ATCCGAGCCAAGGAGTGTGTCTCCATACAAGCTTGCCATTGCAGCCAGCACGAACCTTAC
TACCCCCAACAGCAAGCTCAACATACCACATGTCTGGATTCATCTGCCAAAGAACAAAAC
CTCCAGTCTCAGCCTTAGATGGGTTTCGATTCCTAACGGTTCTTGGTAGCAG

Last Updated on 06.12.2018 16:11