Sequences from confirmation amplicon


Confirmation sequences for line 705G05 and hit At3g52880 are shown.
Line ID 705G05
Locus At3g52880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19601524
Primers used for PCR GE55 - 8474, show primer details
   
Sequence Name 82-K101222-0022-705-G05C-8409
GenBank Accession LN514160 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K101222-0022-705-G05C-8409
GACTCTCTGCGAAGGTCGAGCTTTGGCGACTTTAGCCAAGGCTTTGTTCTCATCACCACT
ACCTCCCTCCATGAATGCTCCAACCACTTTACCACCTTGAACCCAATATGCTCCAAATCT
TGGTTTTGGGTTTGATGGATTGCTGTCTCCAAATAACACAGAGTCTCCTACGTTGTCTCC
GTAGAACTGCCATGAGAGATCAAAGGAGCGAGAGTAGAAGAATGGAAGATAGTCGTATTC
CTCCACCGCTGCGCCTCCCTCAGCCGCTTTGATCGCCTGCCACAATGTATCACATATATA
AACGCTTCATCAGATATATACAACTGTATGTTAATCTATAAGATTTAGGCCTCAAGAGTT
TGCGTTGCATTTTTTATGGTATGGATTAATAGGGTTTTGGTGTAGCGATATCAGCCATAT
TGTAAGCATTATTTACCTTAACAGCTTGCTCTGCTGATTTGCGGGAATGGTCAACATGCT
CGACCCTTCTCACGTCTCCGTACATTTTCAAAGGGAAAGTAGCAACGTCACCAACAGCGT
ANACATCTGGAACACNTTGTTTTGAA
   
Sequence Name 82-K101229-0022-705-G05C-GE55
GenBank Accession LN514161 [GenBank]
Sequencing primer gene specific primer, GE55: 5'-TCTTCAAAACAAGTGTTCCAGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K101229-0022-705-G05C-GE55
TCTTTCCTTTGAAATGTACGGAGACGTGAGAAGGGTCGAGCATGTTGACCATTCCCGCAA
ATCAGCAGAGCAAGCTGTTAAGGTAAATAATGCTTACAATATGGCTGATATCGCTACACC
AAAACCCTATTAATCCATACCATAAAAAATGCAACGCAAACTCTTGAGGCCTAAATCTTA
TAGATTAACATACAGTTGTATATATCTGATGAAGCGTTTATATATGTGATACATTGTGGC
AGGCGATCAAAGCGGCTGAGGGAGGCGCAGCGGTGGAGGAATACGACTATCTTCCATTCT
TCTACTCTCGCTCCTTTGATCTCTCATGGCAGTTCTACGGAGACAACGTAGGAGACTCTG
TGTTATTTGGAGACAGCAATCCATCAAACCCAAAACCAAGATTTGGAGCATATTGGGTTC
AAGGTGGTAAAGTGGTTGGAGCATTCATGGAGGGAGGTAGTGGTGATGAGAACAAAGCCT
TGGCTAAAGTCGCCAAAGCTCGACCTTCTGCAGAGAGTCTCGACGAGCTCGTGAAACAAG
GAATTTCCTTCGCAGCANTGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATNTCCGCA

Last Updated on 06.12.2018 16:11