Sequences from confirmation amplicon


Confirmation sequences for line 705G10 and hit At1g53440 are shown.
Line ID 705G10
Locus At1g53440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19946884
Primers used for PCR JV55 - 8474, show primer details
   
Sequence Name 13-K101423-0022-705-G10C-8409
GenBank Accession LN491971 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K101423-0022-705-G10C-8409
ATTTACATTGAAATATCCTGTCGCCACAAACATGTAACAAAGCCCCTTTTTCTGTCCAAC
AAGAAGTTCTTTCGATGTTCACTGTTTGGTTTTGAAGCTTTCTAAAGATCGTTCGCAATG
TTTGAACTGTGCAAAATTGGTCAAACAATTTGTCGGTTGAGAATTAAAGTAAGAACTTTG
ATATAATAAATAATGAAAGTCCAACAAAATGAATAAGAATGAGAGATAAATACTATACAA
TAAAAAACATTTCTGTCTCAACGTCTCTATCGATCTTTTAAGTAAAATTTTTGACCTAAC
TGGCCAAATTGCACATGTATTGAAATACAATTATTAATAAGAAGACACGGGCTTACTGTT
   
Sequence Name 53-K101426-0022-705-G10C-JV55
GenBank Accession LN508095 [GenBank]
Sequencing primer gene specific primer, JV55: 5'-AAACAGTAAGCCCGTGTCTTCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K101426-0022-705-G10C-JV55
GTGCATTTGGCCGTTAGGTCAAAAATTTTACTTAAAAGATCGATAGAGACCTTTAGACAG
AAATGTTTTTTATTGTATAGTATTTATCTCTCATTCTTATTCATTTTGTTGGACTTTCAT
TATTTATTATATCAAAGTTCTTACTTTAATTCTCAACCGACAAATTGTTTGACCAATTTT
GCACAGTTCAAACATTGCGAACGATCTTTAGAAAGCTTCAAAACCAAACAGTGAACATCG
AAAGAACTTCTTGTTTGGACAGAAAATGGAACTTTGTTACATGTTTGTTGGCGACAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAG
ATGTCCGC

Last Updated on 06.12.2018 16:11