Sequences from confirmation amplicon


Confirmation sequences for line 706E09 and hit At2g19010 are shown.
Line ID 706E09
Locus At2g19010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8244952
Primers used for PCR KA04 - 8474, show primer details
   
Sequence Name 49-K105356-0022-706-E09C-KA04
GenBank Accession LN506839 [GenBank]
Sequencing primer gene specific primer, KA04: 5'-TGTCAAAGCCGTACAACACTAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K105356-0022-706-E09C-KA04
CATATTCTCTCATTATTATTTACCGTTCCCACTTAAAGGTAAAGTGGAACCATTTGGTGT
GACATATATTTAGGATAATCATTCATTGTTTTTATTTTTACTAGTTATTTAAATTAGTTT
GGTTTTTAGGGTTATCTAACGGTGTTCAGGTTATCAAGTTTTCTTCTAAATAACCAAATT
ATGTGCATGCTTGTAGAATTTGCACCGTCTAGGAGCGAGGAAGGTGGCAGTTTTCGGACT
CAGCCAGATTGGGTGCACACCAAAGATAATGAAGTCACACAGTGACGGTAAAATCTGTTC
CCGCGAAGTGAATGAAGCAGTGAAGATTTTTAATAAGAACCTTGACGACCTACATGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCG
   
Sequence Name 33-K105357-0022-706-E09C-8409
GenBank Accession LN500993 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K105357-0022-706-E09C-8409
CATTTACAATTGAATATATCCATGTAGGTCGTCAAGGTTCTTATTAAAAATCTTCACTGC
TTCATTCACTTCGCGGGAACAGATTTTACCGTCACTGTGTGACTTCATTATCTTTGGTGT
GCACCCAATCTGGCTGAGTCCGAAAACTGCCACCTTCCTCGCTCCTAGACGGTGCAAATT
CTACAAGCATGCACATAATTTGGTTATTTAGAAGAAAACTTGATAACCTGAACACCGTTA
GATAACCCTAAAAACCAAACTAATTTAAATAACTAGTAAAAATAAAAACAATGAATGATT
ATCCTAAATATATGTCACACCAAATGGTTCCACTTTACCTTTAAGTGGGAACGGTAAATA
ATAATGAGAGAATATGCGTATTGTTTAGGAGTGTAGCGTCGTTTAGTGTTGTACGGCCTT
TGAC

Last Updated on 06.12.2018 16:11