Sequences from confirmation amplicon


Confirmation sequences for line 707C05 and hit At5g51870 are shown.
Line ID 707C05
Locus At5g51870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21087641
Primers used for PCR TN01 - 8474, show primer details
   
Sequence Name 27-K117188-0022-707-C05C-TN01
GenBank Accession LN498569 [GenBank]
Sequencing primer gene specific primer, TN01: 5'-GGGTGTGTGGGTAACAGTTTAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117188-0022-707-C05C-TN01
GAACTTTTTTTCGAAACCCTAATACAATCCACTTGCTCGTGCTTGAAGAAACTCTCTTAA
GTAGTAAAAATTCTTAAGTTTGCTTAAATAAAATCCAGAAGCTAAGTACTTACAAAACTT
AAATTCTAAAATTAGAATGTGACAAGGCATTGTTGGGCAAAGTCAGGAACTAGCTAGCAC
CAAAAGAAAGGGGGCCAAATCTTTAGCGCCTATATTATTGCAAAATCTCTAGTACGATTT
AACCAGAGATATATTATACTATTCACTACAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATTATAAT
   
Sequence Name 12-K117187-0022-707-C05C-8409
GenBank Accession LN491764 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117187-0022-707-C05C-8409
ATTTACATTGAATATATCCTGTAGTGAATAGTATAATATATCTCTGGTTAAATCGTACTA
GAGATTTTGCAATAATATAGGCGCTAAAGATTTGGCCCCCTTTCTTTTGGTGCTAGCTAG
TTCCTGACTTTGCCCAACAATGCCTTGTCACATTCTAATTTTAGAATTTAAGTTTTGTAA
GTACTTAGCTTCTGGATTTTATTTAAGCAAACTTAAGAATTTTTACTACTTAAGAGAGTT
TCTTCAAGCACGAGCAAGTGGATTGTATTAGGGTTTTCAGAAAAAAAGTTTCTTGAACAG
TGAACCTCGTATTCCTTGGAACTTAAACTGTTACCCACACACCC

Last Updated on 06.12.2018 16:11