Sequences from confirmation amplicon


Confirmation sequences for line 708B08 and hit At2g25260 are shown.
Line ID 708B08
Locus At2g25260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10757413
Primers used for PCR 26pq - 8474, show primer details
   
Sequence Name 52-K115259-0022-708-B08C-26pq
GenBank Accession LN507909 [GenBank]
Sequencing primer gene specific primer, 26pq: 5'-TTTAGAACAACATATCCCTGCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K115259-0022-708-B08C-26pq
TTTTTTTGTGTATATTTCTTTAGAAAATTTCATCATCCTTGGAATAAAATTTACACATAT
TATCATTTGTTTTACGAGGTTTTTTCATGTTTGTTTTAAATCTTATAATTTCATAAAAGA
TGCTTATGTTTATTAAAAATAATGTTTTAATTTTTTTTTGATAAAGAAGAAGTTTTATCC
TCAACATGATTTCGTGAACATGCATTACTTATTTTGACAAGTCAATCAATAATATAATAC
AAGATTTGAGATTGTTTTTTTTTACGTTACTATTTTCAATTATAAAATAAACCTCTAAAT
CGAAAAATAACATCATGATGATTCTAAAATCTAGCTATAACCAATACACCTTTCACCAAT
AACAAAGAATTAAGTAAGATAAAGAACCTTGTCAACACCGGAGGGAAGAGGGTCAGCAAC
GAAAGTAGGGATCTACATGAACCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTC
CGCACGAT
   
Sequence Name 12-K115258-0022-708-B08C-8409
GenBank Accession LN491706 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K115258-0022-708-B08C-8409
TACTTTCGTTGCTGACCCTCTTCCCTCCGGTGTTGACAAGGTTCTTTATCTTACTTAATT
CTTTGTTATTGGTGAAAGGTGTATTGGTTATAGCTAGATTTTAGAATCATCATGATGTTA
TTTTTCGATTTAGAGGTTTATTTTATAATTGAAAATAGTAACGTAAAAAAAAACAATCTC
AAATCTTGTATTATATTATTGATTGACTTGTCAAAATAAGTAATGCATGTTCACGAAATC
ATGTTGAGGATAAAACTTCTTCTTTATCAAAAAAAAATTAAAACATTATTTTTAATAAAC
ATAAGCATCTTTTATGAAATTATAAGATTTAAAACAAACATGAAAAAACCTCGTAAAACA
AATGATAATATGTGTAAATTTTATTCCAAGGATGATGAAATTTTCTTAAAGAAATATTTA
CACAAAAAAAACATGTGATGATGAATTGTATATAAGGTGCAGGGATATTTGGTTCTAA

Last Updated on 06.12.2018 16:11