Sequences from confirmation amplicon


Confirmation sequences for line 708D04 and hit At4g05060 are shown.
Line ID 708D04
Locus At4g05060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2591693
Primers used for PCR 76ys - 8474, show primer details
   
Sequence Name 57-K122490-0022-708-D04C-76ys
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 76ys: 5'-TAAGTTTCAAGCAGCGTCTACTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K122490-0022-708-D04C-76ys
TCATTAGTACAGAAGATAAACACAGTTAACATCAGTATCACACTTTTCTTTGTTGTCTTA
TAAAACAAAACATAAAAAGAAGACGAGAGTAAGGCATGAAAAGAATGGCATTCGTCTCTC
ACCCATTCATCGATCACAAGCCCTTCTCCAATTGGCTTTGGACCAACAATACCCTCTGAT
GCTTTTTTCCGTGCTTCATCTGCAGCATCCGCTTCAGCCAATTGACTCTTCAATTTCTCC
ATCATCTGTAAAGAGAGAGGTAACAAAGTTTAAAGAATCAGTGATATCTAGGATTGCTTA
AAGCAGTAAATGATCCCAATCAAGTTCACAAATGATGGCGATAAGAACCAGAAAAAGAAG
AAGAAGCGGACGGACTCACGGAGTTAGGGTTTTCGGGATCCAAGAATACCACACGCATTA
CTTGCTCCTCAGAGACATGATCTTTTTGCTCCTCAAACTGCAAAACACAAGAGCATCCAC
ACTTCTTAGAATCCAAGAACAAAAGTCTTATTTTATCTTATCTTCTCTGTTTCTTTCTGA
CCAGTAGTTACTACAAGTTTCACATACCAGCTCAGGCATATAGTCTGTTGGAACTTTCAT
CTTCAAGCTCATGATTTTAAACTTAACCCCGCTCTTTTGTTCCATTGGCTTTTCATTATT
CTCAGGAGGCTCAACAAACTTGAAAACTAAAGAATTCAACAAGACATAGAAACAAACAGT
CACCACATAATCATCGAGGCCAATGATAAAAAGTACCCTAATCAAACAGTATAAAGAAAC
ATATATACAAGAGAAGCAAATACCAGTTGCGATAATTTCTTCCCCGGGAGCAAGAATAGC
ACCAGCCGGGCGCATGAAGCAACTCTTTGGTACTGTTGTTTTGAACTGAGTAAACCNNAG
ATATCAGCAGAGATAAA
   
Sequence Name 25-K122489-0022-708-D04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K122489-0022-708-D04C-8409
ATCCTTCAGCAAAACTTTACTTCCCTTGTGAGTTTCTTCTACTCTTAATCGGATCTCATC
GAAGACTTTGACTGATCAATTCTATAAAATTTCGAATTTTTTTTTGGATCATAGTGTTGA
TATTGACTTAACTGGTTTAGCTTTATCTTTGGGAATCATGAAATTGGATATATATTGAAG
GTGATGATGATGTTGACTTGTAATTTGACTTATTGATACGTTACTATAAACTGTATAAAG
CCATTGAGCAAATTTAGGAATAAAATGTGGAGTCTTGTCAATTTCAAGATTCACTCTTTA
TTGATGATCAAAGATTAGTGATTTAGTTTATGAACTTTGTATGTAGATGAGCCTGGGAAG
CAAGTCAGGAGTGCCATTAAGATTAAGAATACGAGCAAGTCACATGTAGCGTTTAAGGTT
TGTAATGGATTCAAAAACGTTTTGATTATGGTTGTTGTTGTTGTCATTGGTGTTTATCTC
TGCTGATTATCTTGGTTTACTCAGTTTCAAACAACAGTACCAAAGAGTTGCTTCATGCGC
CCGGCTGGTGCTATTCTTGCTCCCGGGGAAGAAATTATCGCAACTGGTATTTGCTTCTCT
TGTATATATGTTTCTTTATACTGTTTGATTAGGGTACTTTTTATCATTGGCCTCGATGAT
TATGTGGTGACTGTTTGTTTCTATGTCTTGTTGAATTCTTTAGTTTTCAAGTTTGTTGAG
CCTCCTGAGAATAATGAAAAGCCAATGGAACAAAAGAGCGGGGTTAAGTTTAAATCATGA
GCT

Last Updated on 06.12.2018 16:11