Sequences from confirmation amplicon


Confirmation sequences for line 709A03 and hit At5g26300 are shown.
Line ID 709A03
Locus At5g26300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9229629
Primers used for PCR CR38 - 8474, show primer details
   
Sequence Name 28-K107199-0022-709-A03C-CR38
GenBank Accession LN498871 [GenBank]
Sequencing primer gene specific primer, CR38: 5'-AAGAAAATGGATGATCAGAAATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K107199-0022-709-A03C-CR38
GCTTACTCTTCCACTTCCTCTTCGCTGAGTTTCTCATTCACAGGTACAGTTTTGTTTGTT
TTTTTCCCTTTTTCGAAACACTTTTTTGATTGATTGGCTTATTACTAAAAGTAGCTTTTT
ACAAAATCCAGGTCACAGGGGGCAGAGGAGCAGAGTACAACAGTTCTTACAGTCTCAAGG
AGAATTTTGGTACACTTGTTGTTATGAAATCTTCTTTCTTTAGATTTCTTGGTTTACAAA
AAAAAAAAAAAAAAAAAAAACGGAACCCCCTTTTTGGGAATTTTTTTTTTTGAAGGGGGG
GAAAAAAGTTTTAAAAAAAAACCCCCCCCCGGAAAAAATTTTAAAAAAAAAAAATTTTTT
TGGAAAAAAGGGCTTTTGGGCGGGGAAATTCCCGGGGGACCCCAAGGGATTTAGGGGGCA
ATTGGGGAAAAAAAAAAAAAAATTTCCCCTTTTTTTTTTCTTTCAAAAAGGGGATTTCCC
CCCCCGTTTTTAAAAA
   
Sequence Name 12-K107198-0022-709-A03C-8409
GenBank Accession LN491603 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K107198-0022-709-A03C-8409
TCACAGAGAAGTTTGTTATTGTCAGAATCTTACTCGATGGTCGCTCATCTCTCAACACTC
TTGTCCCTCCTGTCAACACAAAAAGTTCCAAGAAGTGGGTTTCAGTTTTTTTTTTTTTTT
TTTTTTTTGGAAACCCAAAAAACCAAAAAAAAAAAATTTTTAAAAAAAAAGGGGCCCAAA
ATTCCCCCTAAAAAGGAAAAAAATTGTTTACCCCCCCCCCCCCCCCCCCGGAACCCGGAA
TTTTAAAAAAACCCCTTTTAAAAAAAACCCCAACAAAAAAAAAAGGTTTTCAAAAAAGGG
AAAAAAAAAAAAAAAACTTTCCCCTTTAAAAAAAAAACCCCCCCAAAAAAAAAGGGGGAA
AAAAAAAAACAAAAAATTAAAAAACCCAACCCCCTTTTTAAACCCCCCTTTTAAAAAA

Last Updated on 06.12.2018 16:11