Sequences from confirmation amplicon


Confirmation sequences for line 709D01 and hit At5g62970 are shown.
Line ID 709D01
Locus At5g62970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25272451
Primers used for PCR 48uw - 8474, show primer details
   
Sequence Name 39-K117737-0022-709-D01C-48uw
GenBank Accession LN503502 [GenBank]
Sequencing primer gene specific primer, 48uw: 5'-GATATGGACAAGATCAGTGGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K117737-0022-709-D01C-48uw
CGTTTCTTCCATTTAATTTGCTATAACAACGAGTGTTTTGTCGAAGCAATGGAAGTTTCT
TTGGATGCGGGTGCCAAAACTTGAGTACGATGAAGATAGCATGTATTCTTTCGAATATTC
CTTCCGATATTTTTTACCTAAGGCTAAGGAGGTTGACAGCGAAACCTATTCTATAGTGTC
CGAAAGCGGCCATAGGATGCGGTCTTTTATTGAGAAGAATCTGCCATTACATAGCTCTCC
GGTCATAGAGAGCTTGCGTCTCAAGTTTTTCACTGAGGTATTTCAACCTGAAGATATCAA
ATTGTGGGTTGAAATTGCAGTTTCTCGTTGTGCTCAAGAGCTAAGCGTTGACTTTTTTCC
CAAGGACCGACACTGTAATGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCACGATTATTA
   
Sequence Name 07-K117736-0022-709-D01C-8409
GenBank Accession LN489456 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K117736-0022-709-D01C-8409
TCGGACATGAGCCATTTACAATTGAATATCATTACAGTGTCGGTCCTTGGGAAAAAAGTC
AACGCTTAGCTCTTGAGCACAACGAGAAACTGCAATTTCAACCCACAATTTGATATCTTC
AGGTTGAAATACCTCAGTGAAAAACTTGAGACGCAAGCTCTCTATGACCGGAGAGCTATG
TAATGGCAGATTCTTCTCAATAAAAGACCGCATCCTATGGCCGCTTTCGGACACTATAGA
ATAGGTTTCGCTGTCAACCTCCTTAGCCTTAGGTAAAAAATATCGGAAGGAATATTCGAA
AGAATACATGCTATCTTCATCGTACTCAAGTTTTGGCACCCGCATCCAAAGAAACTTCCA
TTGCTTCGACAAAACACTCGTTGTTATAGCAAATTTAAATGGAAGAAACGATAAGATCTT
CACGAGCAATTCATCATCAGAAAACCCACTGATCTTGTCCATATC

Last Updated on 06.12.2018 16:11