Sequences from confirmation amplicon


Confirmation sequences for line 709G04 and hit At5g57690 are shown.
Line ID 709G04
Locus At5g57690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23373862
Primers used for PCR 23bw - 8474, show primer details
   
Sequence Name 88-K107938-0022-709-G04C-23bw
GenBank Accession LN514873 [GenBank]
Sequencing primer gene specific primer, 23bw: 5'-GAGCTTCGTCAAAGAGTTATGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K107938-0022-709-G04C-23bw
GCGATTGCTTCTTCGTAAGACGATTCCTTCGACGCTTCCTCCTCTGTAGCACCACCGTTA
GAAAACAACGCGTTGACGCCTGAAGTTCCTTTGATGGTCTTCGTTAATCCCAAAAGCGGT
GGTCGTCAAGGTCCACTCATTAAAGAACGTCTCCAGAATCTTATTAGCGAAGAACAGGTG
TATGATTTAACGGAAGTGAAGCCTAATGAGTTTATTCGATATGGATTGGGATGCTTAGAG
GCATTTGCGTCTCGAGGAGACGAATGTGCTAAAGAGATTCGTGAAAAGATGAGAATTGTG
GTACTTTTTTCCATTGCTTGATCTACAATCAAAGATTTGAAAATGTGTTTTTTTTTTTGT
TATTGCCACGGGTTTGATCACTCATTACAGGTTGCAGGTGGAAATGGAACCGTTGGTTGG
GTTCTAGGTTGCAGGATATATTCAATTGTAAAGGGCTTCATGTCCGGGAAATCTACTTGG
ATCACCAATGAGTATGATGGTCAATATGGAGAAAAAAAAAGAGTAATTCCCAATTTTTTT
TCATTTCAAAAATGTAAATGTCCCCACCGTTATTAA
   
Sequence Name 79-K107937-0022-709-G04C-8409
GenBank Accession LN513834 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K107937-0022-709-G04C-8409
CGGAATGAGCCATTTACATTGAATAATCCTGCAACCTAGAACCCACCAACGGTTCCATCT
CCTCCTGCAACCTGTAATGAGTGATCAAACCAGTGGCAATAACAAAAAAAAAAACCCTTT
TTCAAATCTTTGATTGTAAATCAAGCAAGGGAAAAAAGTCCCACAATTCTCATCTTTTCA
CAAATCTCTTTAGCACATTCGTCTCCTCGAAACGCAAATGCCTCTAAGCATCCCAATCCA
TATCGAAAAAACTCTTTAGGCTTCACTTCCGTTAAATCATACCCCGGTTCTTCCCTAATA
AAATTCGGGAAACGTTCTTTAAGGAGGGGACCTTGACAACCCCCGCTTTTGGGATTAACG
AAAACCATCAAAGGAACTTCGGGCGTCAACGCGTTGTTTTCTAACGGGGGGGCTACAAAG
GAGGAAGCGCCAAAGGAATCGTCTTTACAAAAAATGCAATCCCGTATTGCAAGGCGGAAA
TATTGAGGAAGCATAACTCTTTGACAAAC

Last Updated on 06.12.2018 16:11