Sequences from confirmation amplicon


Confirmation sequences for line 710G05 and hit At2g18876 are shown.
Line ID 710G05
Locus At2g18876 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8170755
Primers used for PCR CR41 - 8474, show primer details
   
Sequence Name 29-K107199-0022-710-G05C-CR41
GenBank Accession LN499314 [GenBank]
Sequencing primer gene specific primer, CR41: 5'-AAATCAGAGTCAGTTTTCTTCCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K107199-0022-710-G05C-CR41
TATTTACAGGAAAATGTTCATTAGCGAAAGAGGATAGTCTAAGAAAACTCGATAATAGTT
ATTTCAAATCGCATATAGTACCTGGAGTAGATTCATAATCTCCATGCCTGATCTCGTTTC
CTTCTTTTTCTCCATCAACACTTGGTTTAACCTCTCCTAAAACAAGATCAATTTTTAATT
AATTAAGATTGAACCTATATTACAAACACTCTGAACTTGCTATGTGAACCCATACCTGTA
ACTTTATGTACTCTTTTTCTTTCTTTTTTGTTTCATGTAATTGTTGGGTCTTGACTTGCT
TTCATACACAAACAATAACAAAATGTTCAAAAAGCATTGCATCAGCATTCCCAAATTCTT
TGAAACATGATCCGCATCACAAAAAAACTGAGAATTCGAGCTGGTATTAAGCAACGATTC
AAACCTGGTTAGCAATCACCATTCGTTGAAACTCATCTCTCTCTTTTTGAAGCTTCTCAT
TCTGCGTCTTTAAAGCCGCTGTATTTTTTGCGTCTTCAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 13-K107198-0022-710-G05C-8409
GenBank Accession LN492065 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K107198-0022-710-G05C-8409
CGGAATGAGCCATTTACAATTGAATATATCCTGAAGACGCAAAAAATACAGCGGCTTTAA
AGACGCAGAATGAGAAGCTTCAAAAAGAGAGAGATGAGTTTCAACGAATGGTGATTGCTA
ACCAGGTTTGAATCGTTGCTTAATACCAGCTCGAATTCTCAGTTTTTTTGTGATGCGGAT
CATGTTTCAAAGAATTTGGGAATGCTGATGCAATGCTTTTTGAACATTTTGTTATTGTTT
GTGTATGAAAGCAAGTCAAGACCCAACAATTACATGAAACAAAAAAGAAAGAAAAAGAGT
ACATAAAGTTACAGGTATGGGTTCACATAGCAAGTTCAGAGTGTTTGTAATATAGGTTCA
ATCTTAATTAATTAAAAATTGATCTTGTTTTAGGAGAGGTTAAACCAAGTGTTGATGGAG
AAAAAGAAGGAAACGAGATCAGGCATGGAGATTATGAATCTACTCCAGGTACTATATGCG
ATTTGAAATAACTATTATCGAGTTTTCTTAGACTATCCTCTTTCGCTAATGAACATTTTC
CTGTAAATATATGCACAGAAAGAAGGGAGACAGCGTGGAACATGGAGTGGGAAGAA

Last Updated on 06.12.2018 16:11