Sequences from confirmation amplicon


Confirmation sequences for line 710G06 and hit At2g16780 are shown.
Line ID 710G06
Locus At2g16780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7282931
Primers used for PCR NK38 - 8474, show primer details
   
Sequence Name 51-K103393-0022-710-G06C-NK38
GenBank Accession LN507472 [GenBank]
Sequencing primer gene specific primer, NK38: 5'-GAGGAAGATTTTTCTGTGTGGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K103393-0022-710-G06C-NK38
TCTTTTGATCTCTCCCCACTCGAATGGCCATCTCTCACCGTCCATTGGGTTCCATCGACG
CCAAATCCTTACGTAGCAGACTCTTACTTCGGTGTCCACAAGCTTATACTCGGTACTCAT
ACTTCCGGTAGTGCTCAAGATTTTCTCATGGTCGCCGACGTCGTTACCCCGACACCAAAC
GCAGAACCTGGGATAGGCGGCGCTNATCAGGATCCCTTTATTCCTAAGGTATTTGATCCC
TGGAGATTCTTCGTTTTGTTGGACAATATTTATAGCTCATGTTTTACGCGCACCAAGTGT
TCGATCAAATGACTAGCTAAAACTGATAGTGTTAAGGATTAGTTAGTTATAGGATTTGAG
ATATTAAATTTGGACTCTTTTATAGGTTTAGGATAAAGAAGAGTTTTTGTATACTACTAA
ACCATGTTTCTTCAATTTTCTGATTTGTTTCAGGTGGAGATTAGGCAGAGGATACGTGTT
GATGGAGAAGTGAACAGAGCGCGGTGTATGCCTCANAAGCCGACGCTGGTGGGTGCAAAG
ACAAGTGGGTGTGAGGTGTTTCTATATTGGATTTCCNGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTCCAATTCANAAATGTAGA
   
Sequence Name 41-K103394-0022-710-G06C-8409
GenBank Accession LN504046 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K103394-0022-710-G06C-8409
GAGCCTTTACAATTGAATATATCCTGGAAATCCATATAGAAACACCTCACACCCACTCGT
CTTTGCACCCACAAGCGTCGGCTTTTGAGGCATACACCGCGCTCTGTTCACTTCTCCATC
AACACGTATCCTCTGCCTAATCTCCACCTGAAACAAATCAGAAAATTGAAGAAACATGGT
TTAGTAGTATACAAAAACTCTTCTTTATCCTAAACCTATAAAAGAGTCCAAATTTAATAT
CTCAAATCCTATAACTAACTAATCCTTAACACTATCAGTTTCAGCTAGTCATTTGATCGA
ACACTTGGTGCGCGTAAAACATGAGCTATAAATATTGTCCAACAAAACGAAGAATCTCCN
GGGATCAAATACCTTAGGAATAAAGGGATCCTGATTAGCGCCGCCTATCCCAGGTTCTGC
GTTTGGTGTCGGGGTAACGACGTCGGCAACCATGAGAAAATCTTGAGCACTACCGGAAGT
ATGAGTACCGAGTATAAGCTTGTGGACACCGAANTAAGAGTCTGCTACGTAAGGATTTGG
CGTCGATGGAACCCAATGGACGGTGAGAGATGGCCATTCGAGTGGGTGAGAGATCAAAAG
ATCGTAGAGAAATGGTGTGTTCTTCTTCCACACAGAAAAATCTTCC

Last Updated on 06.12.2018 16:11