Sequences from confirmation amplicon


Confirmation sequences for line 710H08 and hit At4g20930 are shown.
Line ID 710H08
Locus At4g20930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11201078
Primers used for PCR 26yz - 8474, show primer details
   
Sequence Name 33-K123356-0022-710-H08C-26yz
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 26yz: 5'-GCAGCTAATCATACAGCTAACAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K123356-0022-710-H08C-26yz
AACTATAGCTAAGGTAATAACTTACATGAGATGATGAAGGCAACATTGTAATCACAACTT
CGCTATCTTGTGCAACTTCATAAGGAGTTTCTCTAGAAGAGACTCCCATTTCAGTGAACA
TTTTCATAACATCACGGTTTCTGAAAATTCAAACACAAGTATATGCACAAGAAAGTTAAT
CAAAGGAATCAACATTAACCAAATATGAAAATTGATAGGCAGCAAAACGCACTCAAAGTA
CATTACATGTCATGAACGGTAACTTTATATCCAGCTCTAATTAGATTATTCACCATTCTG
AATCCCATATTTCCAAGTCCTATGAAACCAACATTCTGACAAAACAGCAAAAATGAGACT
TCATTTACATAAATTTTGGGTACTGAATCAGACAAATTTTGCTGTTAAAAATCTAATCCA
GACAACTTTTTTTGCAGAACTGATGAATTTAGATAAGTGGTCAAGATCAATAAGCAATTT
GGCCACTAAAATTCTTATTAACTATGCTCGAGATTACAAAAGTTAAGATCTTTTATTCCA
ACTACCTTAAGGATCATGTTTTAGAACGTTTCAATAAAATTCTAGGTGACTTATGAGGAA
AAGAGATTATGTTTTCTGATCATGAAGACAATTTAAAAAGCAGAACCTGGAATTGATTTG
AATTTTGAGACGAAGAAGAGAACCGGTGGAGCGACCCAGACACGAAATTGGTTTTGAATT
TAGAAAGACAAAGTAGAGTTTGCGCTCTGCGAATCGCCATTGATGAATTTCTTCAAACTC
AGAGCAAGTTAACAATTAGCAATTAACAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAATGTAGATGTC
   
Sequence Name 16-K123355-0022-710-H08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K123355-0022-710-H08C-8409
CATTTACATTGTTAATTGCTAATTGTTAACTTGCTCTGAGTTTGAAGAAATTCATCAATG
GCGATTCGCAGAGCGCAAACTCTACTTTGTCTTTCTAAATTCAAAACCAATTTCGTGTCT
GGGTCGCTCCACCGGTTCTCTTCTTCGTCTCAAAATTCAAATCAATTCCAGGTTCTGCTT
TTTAAATTGTCTTCATGATCAGAAAACATAATCTCTTTTCCTCATAAGTCACCTAGAATT
TTATTGAAACGTTCTAAAACATGATCCTTAAGGTAGTTGGAATAAAAGATCTTAACTTTT
GTAATCTCGAGCATAGTTAATAAGAATTTTAGTGGCCAAATTGCTTATTGATCTTGACCA
CTTATCTAAATTCATCAGTTCTGCAAAAAAAGTTGTCTGGATTAGATTTTTAACAGCAAA
ATTTGTCTGATTCAGTACCCAAAATTTATGTAAATGAAGTCTCATTTTTGCTGTTTTGTC
AGAATGTTGGTTTCATAGGACTTGGAAATATGGGATTCAGAATGGTGAATAATCTAATTA
GAGCTGGATATAAAGTTACCGTTCATGACATGTAATGTACTTTGAGTGCGTTTTGCTGCC
TATCAATTTTCATATTTGGTTAATGTTGATTCCTTTGATTAACTTTCTTGTGCATATACT
TGTGTTTGAATTTTCAGAAACCGTGATGTTATGAAAATGTTCACTGAAATGGGAGTCTCT
TCTAGAGAAACTCCTTATGAAGTTGCACAAGATAGCGAAGTTGTGATTACAATGTTGCCT
TCATCATCTCATGTAAGTTATTTACCTTTAGCAATAGTTTTATATGAAAACAGTTTGAAA
GATCGTCCTGTTAACTGTATG

Last Updated on 06.12.2018 16:11