Sequences from confirmation amplicon


Confirmation sequences for line 712B06 and hit At2g01880 are shown.
Line ID 712B06
Locus At2g01880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:391802
Primers used for PCR 32vh - 8474, show primer details
   
Sequence Name 64-K117751-0022-712-B06C-32vh
GenBank Accession LN511287 [GenBank]
Sequencing primer gene specific primer, 32vh: 5'-ACTTCACTGAACCAGAAGATCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K117751-0022-712-B06C-32vh
ACCTCTTACTGTAATACATTTTCTTTCATAATCACATATATATATCTCAATCTTCTTTTT
GAAATTTTCTACTAATTATAACTTATGTTATTGAAATATCAGGATTTAGATTTAGAGATT
AAAAAGTCGCGTGCCACATGGAAATTTGTCGTGGGACATCACGGGATCAAAACCGCAGGT
AATCACGGTGTGACTCAAGAGCTTGTAGATCAACTTCTTCCAATTCTAGAGGTAAAATTC
ATAATATCACTTTTATGCTAATTACATGTTATATTATCTTTATTGTTTTCTACATTGATG
TACCCCAATTTAATTAACTACGATAGTTTTTCATGATTTTTTTCACTTATTACTGAAGGA
GAATAAAGTGGACTTGTACATAAATGGACATGATCACTGCTTGCAACACATTGGTTCTCA
CGGGTAAAATTATAAGTACTTATTTGTTGAGTTTAAAATGTTTCTTTCTCTATTAAGTTG
TTATATTTTGTTGAAAGAAAGAAAGCTTAACATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATTC
   
Sequence Name 39-K117750-0022-712-B06C-8409
GenBank Accession LN503503 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K117750-0022-712-B06C-8409
AAGCTTTTCTTTTCTTTCAACAAAATATAACAACTTAATAGAGAAAGAAACATTTTTAAA
CTCAACAAATAAGTACTTATAATTTTTACCCGTGAGAACCAATGTCGTTGCAAGCAGTCG
ATCATGTCCATTTATGTACAAGTCCACTTTATTCTCCTTCAGTAATAAGTGAAAAAAATC
ATGAAAAACTATCGTAGTTAATTAAATTGGGGTACATCAATGTAGAAAACAATAAAGATA
ATATAACATGTAATTAGCATAAAAGTGATATTATGAATTTTACCTCTAGAATTGGAAGAA
GTTGATCTACAAGCTCTTGAGTCACACCGTGATTACCTGCGGTTTTGATCCCGTGATGTC
CCACGACAAATTTCCATGTGGCACGCGACTTTTTAATCTCTAAATCTAAATCCTGATATT
TCAATAACATAAGTTATAATTAGTAGAAAATTTCAAAAAGAAGATTGAGATATATATATG
TGATTATGAAAGAAAATGTATTACATGTAAGAGGTTTGAGATGTATTTATTTCTTGGTAA
GACATTTCTCCAATCATAAGTGTGATC

Last Updated on 06.12.2018 16:11