Sequences from confirmation amplicon


Confirmation sequences for line 712E10 and hit At5g67510 are shown.
Line ID 712E10
Locus At5g67510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26938372
Primers used for PCR 52yh - 8474, show primer details
   
Sequence Name 43-K122428-0022-712-E10C-52yh
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 52yh: 5'-TACCAGTCTATATGTCCGACACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K122428-0022-712-E10C-52yh
ATGAATATCAATAAGGATTTAAATCTCAGTCCAATTTCAATAGCTAGAAATATAAGCATA
AATGAGGTACATTGATAAGAAAACCACAAAATTCAACTTCGAATTCTGAATCATATCATC
ATCAATGAAATCAAAATAGAAAACTAGAAAAGAAGAAGAAAAAACCATACGTTAAGATCA
ATCTACGTTCTCCATGACATCTTCGGCTGAAAACTTGGTTCCCTTCTCCTTATCGGCGGC
GGCGCGTCCATTAGCCTTACGCTCCAAAAGCGACTTCCTATCCTTATCGAGACGGAGCTT
AGTGATCATCACATTCGAAGCATTGACTCCGACGTTGACCGTGGATCCGTTGACTTTCTC
CCTTGTGATTCTCTCGATGTGAATCACCCACTTGCGGCGATACACCTGCATCACCTTCCC
TTCTCTTCCCTTGAACGTCCCACGAACAACTTGCACTTCGTCGTCTTTACGAATCGGCAT
GGATCTGACGTTGTGTTTGTTACGGAGATCCTTGGATAACGGCGAGCTCATGAGAACGCG
CCTCACGCTTGAAGGAGCTGTGAAGTGAGCCTTCCTGTTCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 19-K122427-0022-712-E10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K122427-0022-712-E10C-8409
TTACAATTGAATATATCCTGAACAGGAAGGCTCACTTCACAGCTCCTTCAAGCGTGAGGC
GCGTTCTCATGAGCTCGCCGTTATCCAAGGATCTCCGTAACAAACACAACGTCAGATCCA
TGCCGATTCGTAAAGACGACGAAGTGCAAGTTGTTCGTGGGACGTTCAAGGGAAGAGAAG
GGAAGGTGATGCAGGTGTATCGCCGCAAGTGGGTGATTCACATCGAGAGAATCACAAGGG
AGAAAGTCAACGGATCCACGGTCAACGTCGGAGTCAATGCTTCGAATGTGATGATCACTA
AGCTCCGTCTCGATAAGGATAGGAAGTCGCTTTTGGAGCGTAAGGCTAATGGACGCGCCG
CCGCCGATAAGGAGAAGGGAACCAAGTTTTCAGCCGAAGATGTCATGGAGAACGTAGATT
GATCTTAACGTATGGTTTTTTCTTCTTCTTTTCTAGTTTTCTATTTTGATTTCATTGATG
ATGATATGATTCAGAATTCGAAGTTGAATTTTGTGGTTTTCTTATCAATGTACCTCATTT
ATGCTTATATTTCTAGCTATTGAAATTGGACTGAGATTTAAATCCTTATTGATAATTCAT
CATTCATTTCAATCAATCGTTGTATACGTTAATTCTTCTTGGTGTCGGA

Last Updated on 06.12.2018 16:11