Sequences from confirmation amplicon


Confirmation sequences for line 713E03 and hit At2g35860 are shown.
Line ID 713E03
Locus At2g35860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15061062
Primers used for PCR 25vz - 8474, show primer details
   
Sequence Name 51-K118356-0022-713-E03C-25vz
GenBank Accession LN507636 [GenBank]
Sequencing primer gene specific primer, 25vz: 5'-TTCATCCACACTCTCTTGCATTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K118356-0022-713-E03C-25vz
TCGTCACTTAACCTCTTTAGCCCTGAGATGGGTCGACTCGTGTCAGAAGGCTACGTTTTA
ACCGTTCTTGCTCCTAACGACGAAGCCATGGCTAAGCTCACAACGGACCAGCTTAGCGAG
CCAGGTGCTCCTGAACAAATTATGTATTACCACATCATACCGGAGTATCAAACAGAGGAG
AGTATGTACAACGCTGTTCGGAGATTCGGAAAAGTGAAGTATGATTCATTGAGATTCCCA
CATAAAGTGTTGGCTCAAGAAGCTGATGGATCTGTCAAATTCGGACACGGTGATGGTTCA
GCTTACTTGTTTGATCCTGATATCTACACGGACGGTCGGATTTCAGTTCAGGGTATTGAT
GGAGTCTTGTTCCTATATGAAACGCCGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 51-K118355-0022-713-E03C-8409
GenBank Accession LN507635 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K118355-0022-713-E03C-8409
GGCGTTTCATATAGGAACAAGACTCCATCAATACCCTGAACTGAAATCCGACCGTCCGTG
TAGATATCAGGATCAAACAAGTAAGCTGAACCATCACCGTGTCCGAATTTGACAGATCCA
TCAGCTTCTTGAGCCAACACTTTATGTGGGAATCTCAATGAATCATACTTCACTTTTCCG
AATCTCCGAACAGCGTTGTACATACTCTCCTCTGTTTGATACTCCGGTATGATGTGGTAA
TACATAATTTGTTCAGGAGCACCTGGCTCGCTAAGCTGGTCCGTTGTGAGCTTAGCCATG
GCTTCGTCGTTAGGAGCAAGAACGGTTAAAACGTAGCCTTCTGACACGAGTCGACCCATC
TCAGTGGCTAAAGAGGTTAAGTTGACGAGTATATCAGCCATCTCGTTGTAGCCACCGTAA
TGCAAGAGATGGGGGGAATGAA

Last Updated on 06.12.2018 16:11