Sequences from confirmation amplicon


Confirmation sequences for line 713E07 and hit At2g35660 are shown.
Line ID 713E07
Locus At2g35660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14989438
Primers used for PCR DN47 - 8474, show primer details
   
Sequence Name 33-K101830-0022-713-E07C-8409
GenBank Accession LN500938 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K101830-0022-713-E07C-8409
ATTGAATAATCCTGATAATAGTTTGGTGCATCGTGATGTTTGATAAGCAATAGGTGCTTA
TCACATGTAGTGAGTATCCGTGTAGTGAGTATAAACACATAACCATAGGCTCAGAGCGTG
AGAGAAGAATACCTGTGCAAGCAACCGGGTTCCATCTCCAAGTTGAAGGAGAGTGTCACC
ATTGGCATTGCTTTGTATACTTTCCAGTTTTGAGGAAAACCGAATGGTTTGTGGAGGAAG
CTGGCTTGCAAGTGTTTCCAAGAGTACTCGCCTCTCCACGGCCCGAACTTCTTGGCTATG
CAAGACATACACTTTGGTTAGTTACACACATGAACCAAATGGAATGATTTTAACCAGCAA
GTTATTAAGCAAGCTACCTTTGATCATCGTCTTTGAATTTGAAAGAACGAAGCTCTCTTC
CATCTTCCTTCTTCACTACCATCCTGCTCAGCTGATCATACCAACACACTCAGCTAAAAC
CAAGAACCAATTTTCTCACTTTTTGATATGGGCAGAAAGAAACTCTACCCTTCGATTTCA
AAGAAACT
   
Sequence Name 77-K101833-0022-713-E07C-DN47
GenBank Accession LN513525 [GenBank]
Sequencing primer gene specific primer, DN47: 5'-CAGTTTCTTGAAATCGAAGGGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K101833-0022-713-E07C-DN47
TTGGTTCTTGGTTTAGCTGAGTGTGTTGGTATGATCAGCTGAGCAGGATGGTAGTGAAGA
AGGAAGATGGAAGAGAGCTTCGTTCTTTCAAATTCAAAGACGATGATCAAAGGTAGCTTG
CTTAATAACTTGCTGGTTAAAATCATTCCATTTGGTTCATGTGTGTAACTAACCAAAGTG
TATGTCTTGCATAGCCAAGAAGTTCGGGCCGTGGAGAGGCGAGTACTCTTGGAAACACTT
GCAAGCCAGCTTCCTCCACAAACCATTCGGTTTTCCTCAAAACTGGAAAGTATACAAAGC
AATGCCAATGGTGACACTCTCCTTCAACTTGGAGATGGAACCCGGTTGCTTGCACAGGTA
TTCTTCTCTCACGCTCTGAGCCTATGGTTATGTGTTTATACTCACTACACGGATACTCAC
TACATGTGATAAGCACCTATTGCTTATCAAACATCACGATGCACCAAACTATTATCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCC

Last Updated on 06.12.2018 16:11