Sequences from confirmation amplicon


Confirmation sequences for line 714C09 and hit At4g33390 are shown.
Line ID 714C09
Locus At4g33390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16076690
Primers used for PCR NF43 - 8474, show primer details
   
Sequence Name 16-K102498-0022-714-C09C-8409
GenBank Accession LN493382 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K102498-0022-714-C09C-8409
GCCATTTACAATTGAATATATCCTCATTACAGGAAAAAACTACAGATATACAAAAAGCTG
TTGCTTCTGCAAAGAAAGAACTCGAAGAAGTGAACGCGAATGTGGAGAAAGCAACTTCTG
AAGTGAATTGCTTAAAGGTTGCATCCTCTTCCTTGAGATTGGAAATTGATAAGGAGAAAT
CAGCACTTGACTCATTAAAACAAAGAGAAGGGATGGCATCGGTAACGGTTGCTTCTCTAG
AAGCTGAGATCGACATAACGAGGTGCGAAATAGCTTTGGTTAAGTCCAAGGAAAAAGAAA
CTAGAGAGGAAATGGTGGAGCTACCAAAGCAACTGCAACAAGCAAGTCAAGAGGCAGATG
AAGCGAAATCATTCGCTGAACTTGCTCGTGAAGAGCTGCGGAAGTCTCAAGAAGAAGCAG
AGCAAGCAAAGGCTGGAGCAAGTACAATGGAGAGCAGATTATTTGCAGCTCAGAAAGAAA
TTGAGGCTATTAAAGCTTCAGAGAGGCTGGCATTAGCTGCCATCAAGGCCTTGCAAGAGA
GTGAATCTAGTTCAAAGGAAAACGCTGTTGATTCTCCGAGGACTG
   
Sequence Name 16-K102499-0022-714-C09C-NF43
GenBank Accession LN493383 [GenBank]
Sequencing primer gene specific primer, NF43: 5'-TATTGTCAGTGTTACAGTCCTCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K102499-0022-714-C09C-NF43
ATTCACTCTCTTGCAAGGCCTTGATGGCAGCTAATGCCAGCCTCTCTGAAGCTTTAATAG
CCTCAATTTCTTTCTGAGCTGCAAATAATCTGCTCTCCATTGTACTTGCTCCAGCCTTTG
CTTGCTCTGCTTCTTCTTGAGACTTCCGCAGCTCTTCACGAGCAAGTTCAGCGAATGATT
TCGCTTCATCTGCCTCTTGACTTGCTTGTTGCAGTTGCTTTGGTAGCTCCACCATTTCCT
CTCTAGTTTCTTTTTCCTTGGACTTAACCAAAGCTATTTCGCACCTCGTTATGTCGATCT
CAGCTTCTAGAGAAGCAACCGTTACCGATGCCATCCCTTCTCTTTGTTTTAATGAGTCAA
GTGCTGATTTCTCCTTATCAATTTCCAATCTCAAGGAAGAGGATGCAACCTTTAAGCAAT
TCACTTCAGAAGTTGCTTTCTCCACATTCGCGTTCACTTCTTCGAGTTCTTTCTTTGCAG
AAGCAACAGCTTTTTGTATATCTGTAGTTTTTTCCTGTAATGAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
CGCCCCAA

Last Updated on 06.12.2018 16:11