Sequences from confirmation amplicon


Confirmation sequences for line 714H09 and hit At1g66260 are shown.
Line ID 714H09
Locus At1g66260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24696759
Primers used for PCR 53sm - 8474, show primer details
   
Sequence Name 48-K116491-0022-714-H09C-53sm
GenBank Accession LN506614 [GenBank]
Sequencing primer gene specific primer, 53sm: 5'-TTTCCCTACGTTTCATTTGGTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K116491-0022-714-H09C-53sm
TCTTTGCTTTAGTACTTTCAGCCAATGGGTGTTTTCTCCTCTTTCAAGAAATTTGTTGAT
TAATGTGGCTTCAAGATTCTACAGACCCTTTTAGCTGACTTGATATGTTCTACTGGATCA
GCTTGCCCGCAGGAAGAGGAGCTTGCCATGGCAGAATCAGAATGATTTGTATGAAGAAAC
CCTTAGAGCTGTTGGGGTATCAGGAGTGGAAGTCGGAACCACAGTTTATATTACCAACCT
TGATCAGGGAGTGACAAATGAAGATATAAGGGTATAGCCATTGGTAACAGGATTAGCAGA
GCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACT
AGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTT
GGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCA
   
Sequence Name 16-K116490-0022-714-H09C-8409
GenBank Accession LN493586 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116490-0022-714-H09C-8409
TGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAA
CTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCC
ACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAA
TGGCTATACCCTTATATCTTCATTTGTCACTCCCTGATCAAGGTTGGTAATATAAACTGT
GGTTCCGACTTCCACTCCTGATACCCCAACAGCTCTAAGGGTTTCTTCATACAAATCATT
CTGATTCTGCCATGGCAAGCTCCTCTTCCTGCGGGCAAGCTGATCCAGTAGAACATATCA
AGTCAGCTAAAAGGGTCTGTAGAATCTTGAAGCCACATTAATCAACAAATTTCTTGAAAG
AGGAGAAAACACCCATTTGGCTGAAAGTACTAAAGCAAAGAGCTCCAGGAAGAAGTAGTC
AAGAACCATCCAAGGAGATACCAAATGA

Last Updated on 06.12.2018 16:11