Sequences from confirmation amplicon


Confirmation sequences for line 715G07 and hit At1g70290 are shown.
Line ID 715G07
Locus At1g70290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26471609
Primers used for PCR HP46 - 8474, show primer details
   
Sequence Name 82-K033555-022-715-G07C-HP46
GenBank Accession LN514136 [GenBank]
Sequencing primer gene specific primer, HP46: 5'-AATCTTGGAATAGCAGCTGAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K033555-022-715-G07C-HP46
TGCAGTCTTGTTCTGACTAAACTTGAGAATACTTTTTTATATTTTGTTTTGTGTGTGTTA
GATGGAAGAGCAAAGATGAGTGGGAGACTTGTTATTCGCCTACGGATACAGAGTGGAGGT
CAATGGTGGAACCGGTTATGAGATCGTATATGGAGGCAACAGATGGGACGAGTATAGAGT
TTAAAGAAAGTGCTTTGGTGTGGCACCATCAAGACGCAGATCCTGACTTTGGATCATGTC
AAGCTAAGGAGATGCTTGATCATCTAGAGAGTGTTCTCGCCAATGAGCCTGTGGTTGTCA
AGAGAGGTCAACACATCGTTGAAGTCAAACCACAAGTAAGTATAAAAACAGAGAATCTCT
TTCCCTTCCATCCGCACATTCGATAGTTAGAACGGATCAAAGTTCACTCATTGCCAAGTT
TTGAGTTGGAAAACCATGAAACAAAGAATATGTTTTGACTTGACTCATTGTAATGTATTT
AGGTAAATGGTAAATGGCTTCATGTAAATGGTAATATGTTTTGAGTTGCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTC
TACATTC
   
Sequence Name 82-K033555-022-715-G07C-8409
GenBank Accession LN514135 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K033555-022-715-G07C-8409
CCATTTACAATTGAAATATCCTGCAACTCAAACATATTACCATTTACATGAAGCCATTTA
CCATTTACCTAAATACATTACAATGAGTCAAGTCAAAACATATTCTTTGTTTCATGGTTT
TCCAACTCAAAACTTGGCAATGAGTGAACTTTGATCCGTTCTAACTATCGAATGTGCGGA
TGGAAGGGAAAGAGATTCTCTGTTTTTATACTTACTTGTGGTTTGACTTCAACGATGTGT
TGACCTCTCTTGACAACCACAGGCTCATTGGCGAGAACACTCTCTAGATGATCAAGCATC
TCCTTAGCTTGACATGATCCAAAGTCAGGATCTGCGTCTTGATGGTGCCACACCAAAGCA
CTTTCTTTAAACTCTATACTCGTCCCATCTGTTGCCTCCATATACGATCTCATAACCGGT
TCCACCATTGACCTCCACTCTGTATCCGTAGGCGAATAACAAGTCTCCCACTCATCTTTG
CTCTTCCATCTAACACACACAAAACAAAATATAAAAAAGTATTCTCAAGTTTAGTCAGAA
CAAGACTGCATTTATAATACATACCTAATGAAGTATCCATGTTCAGCTGC

Last Updated on 06.12.2018 16:11