Sequences from confirmation amplicon


Confirmation sequences for line 716G12 and hit At2g41480 are shown.
Line ID 716G12
Locus At2g41480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17297044
Primers used for PCR PF25 - 8474, show primer details
   
Sequence Name 14-K102864-0022-716-G12C-8409
GenBank Accession LN492461 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K102864-0022-716-G12C-8409
GCCATTTACATGGAACTCATCTTAATCATAGCTTTACCAAACTCATAATCAAACCTAAAC
CCTAACAAACCTCTAAGACGACTCGCATACTTCTTCACCACGGCATTTGTCTCGGCGTCC
GACCAAAGCCGTTGGTCCGACTCTAGAATAGCGTTTCCGTCGCGTAAGTTCTTGAAGAAA
CTTTCATCGAATTTTGAGGGGCTACCAATGTCAAGAGCAACACGTTTCGAACCGTCTCCA
TTGGGAGGACAAAGAGTCTTGAGCTGTGTAAGAAACGATGGGCTTATGGTTGGATCGGAG
TTTCCGGTCACCGTGAAGTTGTATAAACGGTAACGGAAGAAGAGACAGTCGGTTTGTCCA
ATTGTATGTGCACCTGATCATAAAATTTAGGTAAGGTAATTGATTGTTGTTCAAAGAAAC
AAAATCCTGGTATGTTCATCAGAAACAACAAGTACAATTTAGTTACTCTTTAAGATGGTC
TAATCGCAAGGGTGTCTTATATTTTCCCTTACAAAACAAGAGTTGTGTCAAATGTTTGTT
AAATTCATGTCTCAACTTCTTAGTATTCTTGATCTATTTGAGTACGACGGCGACNAAAGC
TAGCTACGGCTATNACTTTTATAACTAGTTTTTTCAAAAGTTTCAAAAAAGTATCAACAA
AATGAAACAGGGTTCACTCCAACTTTTAACCACTTGC
   
Sequence Name 14-K102869-0022-716-G12C-PF25
GenBank Accession LN492462 [GenBank]
Sequencing primer gene specific primer, PF25: 5'-TGCAAGTGATTAAAAGTTGGAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K102869-0022-716-G12C-PF25
TTTTTTGAACTTTTGAAAAACTAGCTATAAAAGTAATAGCCGTAGCCTGCATTTTGTCGC
CGTCGTACTCAAATAGATCAAGAATACTAAGAAGTTGAGACATGAATTTAACAAACATTT
GACACAACTCTTGTTTTGTAAGGGAAAATATAAGACACCCTTGCGATTAGACCATCTTAA
AGAGTAACTAAATTGTACTTGTTGTTTCTGATGAACATACCACGATTTTGTTTCTTTGAA
CAACAATCAATTACCTTACCTAACTTTTCTGATCAGGTGCACGTACCATTGGACAAACCG
ACCGTCTCTTCTTCCGTTACCGTTTATACAACTTCACGGTGACCGGAAACTC

Last Updated on 06.12.2018 16:11