Sequences from confirmation amplicon


Confirmation sequences for line 717B07 and hit At5g23270 are shown.
Line ID 717B07
Locus At5g23270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7840813
Primers used for PCR CS15 - 8474, show primer details
   
Sequence Name 72-K030602-022-717-B07C-CS15
GenBank Accession LN512650 [GenBank]
Sequencing primer gene specific primer, CS15: 5'-TTTTGTACCTTCATCCCATTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K030602-022-717-B07C-CS15
TCATTATGTTCTATGCACCTGGTTCTGTTTAAGAACCATCGGTTTTGGAAATGACGCCTC
TCTCATATCCGCAGTTATCACCNGGTCTTGTCAATGTCCTTTCCACCATTGTCTCCATCT
ACTCCGTTGACAAATTCGGACGAAGAGCTTTGTTTCTTCAAGGTGGTTTTCAGATGATCG
TGACCCAGGTGCGTACAAACCCTAAAACAATGAAAATACTCTGTTTTTCAAAACAGAGTA
TCTTGCTCTGCTTTCTAAAACCGAGGTAGGTAACAATGATTTGTTTTGCTTTGGCTTCAG
ATCGCGGTCGGGTCGATGATCGGATGGAAATTTGGATTCAACGGGGAAGGAAATTTGTCA
GGGGTTGATGCGGATATAATCTTGGCACTGATATGTCTCTATGTGGCGGGTTTCGCGTGG
TCATGGGGACCGTTGGGATGGTTAGTGCCGAGTGAGATATGTCCATTGAAAATTAGATCA
GCAGGACAATCGTTAAACGTTT
   
Sequence Name 33-K030602-022-717-B07C-8409
GenBank Accession LN500877 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K030602-022-717-B07C-8409
CATTTACAATTGAATATATCCTGGCAATCAATTGTATCTATGCTCTTTCCACACTTTCCC
CATCTCCTCAATCGGAACACCTTTTGTCTCCGGCAACAAGAAGTAAATGAAAATGGTCAT
GATCAAGACCATACCCGCGAAGAAATAGAACAAACCAAACTTCATATGGCAAAGCATCGT
CAAGAAGAATTGTCCAATGAAGAATGTAAAGAACATGTTCACCGAAACGTTTAACGATTG
TCCTGCTGATCTAATTTCCAATGGACATATCTCACTCGGCACTAACCATCCCAACGGTCC
CCATGACCACGCGAAACCCGCCACATAGAGACATATCAGTGCCAAGATTATATCCGCATC
AACCCCTGACAAATTTCCTTCCCCGTTGAATCCAAATTTCCATCCGATCATCGACCCGAC
CGCGATCTGAAGCCAAAGCAAAACAAATCATTGTTACCTACCTCGGTTTTAGAAAGCAGA
GCAAGATACTCTGTTTTGAAAAACAGAGTATTTTCATTGTTTTAGGGTTTGTACGCACCT
GGGTCACGATCATCT

Last Updated on 06.12.2018 16:11