Sequences from confirmation amplicon


Confirmation sequences for line 717D10 and hit At4g03000 are shown.
Line ID 717D10
Locus At4g03000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1324696
Primers used for PCR 54sm - 8474, show primer details
   
Sequence Name 49-K116491-0022-717-D10C-54sm
GenBank Accession LN506957 [GenBank]
Sequencing primer gene specific primer, 54sm: 5'-CGTGTTTTGTTATTTGGTTGAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K116491-0022-717-D10C-54sm
ATTCATTGCATTCTGATTGAGCTGCTATGTTTGAGAAATCGTGTGTGGAATTGTGTTGAT
TTGTTCTGGGAATTTAATTAGTTTCTGGAATTTTGCCGATTGTTTTGGTGTTTTCATGAA
AAGTTTGCTACTGGAATTTGGGATTATCTCAAAGCTTATTCAATACTTGAATTATTGAAT
CATTTTTGTTATCTCTGTGTTTATAATTTGGAAACAATCATAATCTCAGGGAATTATTGC
TTAATGGTTGAGAAGCAAGAAGAAATGAATGAGTTTGGTGCTGTCAATGGTGGGAAAGTG
GGGACGTCTTCTTCAGTGTCACCACCGCAAGATAAAGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAAGGTTATTAT
   
Sequence Name 17-K116490-0022-717-D10C-8409
GenBank Accession LN494014 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K116490-0022-717-D10C-8409
GCCTTTATCTTGCGGTGGTGACACTGAAGAAGACGTCCCCACTTTCCCACCATTGACAGC
ACCAAACTCATTCATTTCTTCTTGCTTCTCAACCATTAAGCAATAATTCCCTGAGATTAT
GATTGTTTCCAAATTATAAACACAGAGATAACAAAAATGATTCAATAATTCAAGTATTGA
ATAAGCTTTGAGATAATCCCAAATTCCAGTAGCAAACTTTTCATGAAAACACCAAAACAA
TCGGCAAAATTCCAGAAACTAATTAAATTCCCAGAACAAATCAACACAATTCCACACACG
ATTTCTCAAACATAGCAGCTCAATCAGAATGCAATCGAATCAGGATAAAAAACCAAATCG
TTTGATCATTCAACCAAATAAGAAAACACG

Last Updated on 06.12.2018 16:11