Sequences from confirmation amplicon


Confirmation sequences for line 717E09 and hit At2g24260 are shown.
Line ID 717E09
Locus At2g24260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10320498
Primers used for PCR 30uv - 8474, show primer details
   
Sequence Name 27-K117967-0022-717-E09C-30uv
GenBank Accession LN498584 [GenBank]
Sequencing primer gene specific primer, 30uv: 5'-TTGTTTTTCATACGTACAAGGGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117967-0022-717-E09C-30uv
GTTTATTTATTCTCTTCATATAGATGGAAGATTCGTTTTTTTGCCTTTTAGTTTGACATA
TTAAACAAAAAGAAAAGCTGACATATTAGTACAATCTTTACATACCAATATATCAAATTT
ATGGGCCTCTGCAACTAAAATTCCTTATGCTCCCTCCTCAAATTTTATATATACATTCTG
GATTATTATGTCATATAAATACATACATGATAAATAATGTTTTTAAGTTATAAATTAGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGG
TTATTATAAAT
   
Sequence Name 11-K117966-0022-717-E09C-8409
GenBank Accession LN491312 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K117966-0022-717-E09C-8409
ATTTAAACTTAAAAACATTATTTATCATGTATGTATTTATATGACATAATAATCCAGAAT
GTATATATAAAATTTGAGGAGGGAGCATAAGGAATTTTAGTTGCAGAGGCCCATAAATTT
GATATATTGGTATGTAAAGATTGTACTAATATGTCAGCTTTTCTTTTTGTTTAATATGTC
AAACTAAAAGGCAAAAAAACGAATCTTCCATCTATATTGAAGAGAATAAAATAAAACAAA
TTGCACTCTGTAAAAGAATTACCCCTTGTACGTTGGAAAAACAAAAGNNNNNAACAACCA
GTAATTACCAGTTTTTCTTTTATTCAAAAATGGTAGATCTCCCCA

Last Updated on 06.12.2018 16:11