Sequences from confirmation amplicon


Confirmation sequences for line 717F02 and hit At5g47530 are shown.
Line ID 717F02
Locus At5g47530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19282875
Primers used for PCR 55lm - 8474, show primer details
   
Sequence Name 34-K114022-0022-717-F02C-55lm
GenBank Accession LN501495 [GenBank]
Sequencing primer gene specific primer, 55lm: 5'-TCCCTTTTAAAACACTCGAAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K114022-0022-717-F02C-55lm
TACCAATCTTCCTCTCACTTTCTCACCACACACACACACACACACACACACACACACACA
CATACACATAAGAGATATCTATTCTATATATGTATTCATATGCACACTACCTCTGTGTCG
AAGAAATAAAGTGGTGTGTCAAACGCTCACATCGTGAGTGTTGTAAGAGAGCACGAGAGT
CTTTCCTCTTCAACATCTATAACACATCATGTGTATTCTCTTTCTCTCGAACACAAACAA
ATATTAACCCCCACGCGCCCATAGTGACCAAATAAACCAGAACTTTTCACTCTTATCTTT
ACATTAGAGATATCAAAAAATTACCTATACGTGGCCGAGATATATTCATGTGTAAGTGGC
TTCGTGTCGGGGATATCTACGTGGATCAGCAGTGAGTATGATGGTCTATATAGAGAAAAA
GAAAGAGTATTTACCTTTTTTTTTTCAATTCAAAAATGTATATCTCCGCA
   
Sequence Name 13-K114021-0022-717-F02C-8409
GenBank Accession LN492126 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K114021-0022-717-F02C-8409
TTGAATATATCCTGGCCATGTATTGGTAATTTTTTGATATCATTAATGTAAAGATTAGAG
TAAAATGTTCTGGTTTATTTGGTCATTATGGGCTTGTGGTTGTTAATTTTTGTTTGTTTT
CGAGATTAAGTTAATACATTTGATGTTTAATAGTTGTTGAAGTTGAAAGACTCTTGTGCT
CTTTTACAACATTCATGATGTGAGCTTTTGACAAACCATTTTATTTCATCGACAGTGAAG
TAGTGTACATATGAATACATATATCGAATAGATATCTCGTATGTGTATGTGTGTGTGTGT
GTGTGTGTGTGTGTG

Last Updated on 06.12.2018 16:11