Sequences from confirmation amplicon


Confirmation sequences for line 717G09 and hit At5g44070 are shown.
Line ID 717G09
Locus At5g44070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17736722
Primers used for PCR NF17 - 8474, show primer details
   
Sequence Name 70-K102435-0022-717-G09C-8409
GenBank Accession LN512358 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K102435-0022-717-G09C-8409
ATTGGAACTGTTCTGTTGGTTTATGTGACACATGATTTTAATCATAACTTTAATCTACCG
CAGCAATTGGTGCTGAAGGAAGTGCACGAAACTGAACTGTTCAAACACATCAATAAGTTC
TTATCCACAGTGGGTTATGAAGACAGTCTGACTTATGCTGCTGCAAAGGCTTGTTGCCAA
GGAGCTGAAATCTTATCCGGAAGCCCATCAAAAGAGTTTTGTTGTCGGGAAACTTGCGTG
AAATGCATCAAAGGTATGTCGCCATGTCGATTCTTCAAATTTCTTATCATAGTTAAAAGT
TTTGGTGTTAGATCAACTGTTAATTTTTTGTTTCCTATTAAGGTCCTGATGACTCTGAAG
GCACGGTGGTGACTGGAGTTGTGGTGCGTGATGGGAATGAACAAAAGGTTGATCTGTTAG
TGCCATCGACGCAAACTGAGTGTGAATGTGGTCCTGAAGCAACTTATCCAGCAGGAAACG
ATGTGTTCACTGCACTTCTATTGGCTTTACCTCCACA
   
Sequence Name 70-K102436-0022-717-G09C-NF17
GenBank Accession LN512359 [GenBank]
Sequencing primer gene specific primer, NF17: 5'-CTGTGGAGGTAAAGCCAATAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K102436-0022-717-G09C-NF17
TGCTTCAGGACCACATTCACACTCAGTTTGCGTCGATGGCACTAACAGATCAACNCTTTT
GTTCATTCCCATCACGCACCACAACTCCAGTCACCACCGTGCCTTCAGAGTCATCAGGAC
CTTAATAGGAAACAAAAAATTAACAGTTGATCTAACACCAAAACTTTTAACTATGATAAG
AAATTTGAAGAATCGACATGGCGACATACCTTTGATGCATTTCACGCAAGTTTCCCGACA
ACAAAACTCTTTTGATGGGCTTCCGGATAAGATTTCAGCTCCTTGGCAACAAGCCTTTGC
AGCAGCATAAGTCAGACTGTCTTCATAACCCACTGTGGATAAGAACTTATTGATGTGTTT
GAACAGTTCAGTTTCGTGCACTTCCTTCAGCACCAATTGCTGCGGTAGATTAAAGTTATG
ATTAAAATCATGTGTCACATAAACCAACAGAACAGTTTCCAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGGG

Last Updated on 06.12.2018 16:11