Sequences from confirmation amplicon


Confirmation sequences for line 718A01 and hit At1g73810 are shown.
Line ID 718A01
Locus At1g73810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27754984
Primers used for PCR 21an - 8474, show primer details
   
Sequence Name 14-K106971-0022-718-A01C-8409
GenBank Accession LN492528 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K106971-0022-718-A01C-8409
TCGGACTGAGCCTTTACATTGAATTATCCGGACATGGAGAGTGAAGATAGACGAAAGAGT
TGAGGATTGAAAGAGACGTTTCTTATATGGACGTTGAGTATGAAGCCGAGTCCGAATCCG
AAACCGATAAGAAACGAATACAAGACGAGGTTTTGGAAGAATCTATGAGATCTTAATATC
TTGGATATGACGATTTGGTGAAGTTGATGACGACTTTCTTGTTGAAGAGAGTCTTCTTGC
GTTTCTTGGCTCTTTTTCATTATTTTGTGAGTCTGCTGAAACTAACAATTAGAACCGTGG
GACGTTATATAGTCAAGGTTATGAATTTTAAAGAGACTTTTCGTCATTGGGTGTCTTTAA
ATATCTGATCTCACGGGCTTTGGATTTAACTAGAAACATTGAATTGACTTGTACAGGATT
TTAGGGG
   
Sequence Name 30-K106972-0022-718-A01C-21an
GenBank Accession LN499739 [GenBank]
Sequencing primer gene specific primer, 21an: 5'-CCCCTAAAATCCTGTACAAGTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K106972-0022-718-A01C-21an
AAGCCCGTGAGATCAGATATTTAAAGACACCCAATGACGAAAAGTCTCTTTAAAATTCAT
AACCTTGACTATATAACGTCCCACGGTTCTAATTGTTAGTTTCAGCAGACTCACAAAATA
ATGAAAAAGAGCCAAGAAACGCAAGAAGACTCTCTTCAACAAGAAAGTCGTCATCAACTT
CACCAAATCGTCATATCCAAGATATTAAGATCTCATAGATTCTTCCAAAACCTCGTCTTG
TATTCGTTTCTTATCGGTTTCGGATTCGGACTCGGCTTCATACTCAACGTCCATATAAGA
AACGTCTCTTTCAATCCTCAACTCTTTCGTCTATCTTCACTCTCCATGTCCGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGC

Last Updated on 06.12.2018 16:11